拜阿司匹林加维C泡腾片中两种有效成分及有关物质的HPLC法测定

Determination of Two Components and Related Substances in Bayaspirin Plus Vitamin C Effervesent Tablets by HPLC

建立了高效液相色谱法测定拜阿司匹林加维C泡腾片中的2个有效成分（阿司匹林、维生素C）及有关物质（水杨酸、糠醛）。采用HypersilC18色谱柱，以甲醇：5mmol／L四丁基氢氧化铵（以冰乙酸调至pH4．5）为流动相，梯度洗脱，检测波长247n／iq。维生素C、糠醛、阿司匹林和水杨酸分别在24～480、0．5～10、40～800和0．8～16μg／ml浓度范围内线性关系良好，平均回收率分别为100．2％、100．3％、100．2％和99．9％，RSD分别为1．07％、0．85％、0．81％和0．77％。

An HPLC method was established for the determination of two components, aspirin and vitamin C, and their related substances, salicylic acid and furfural in bayaspirin plus vitamin C effervesent tablets. A Hypersil C18 column was used with the mobile phase of methanol ： tetrabutylammonium hydroxide （adjusted to pH 4.5 with acetic acid） by gradient elusion, at the detection wavelength of 247 nm. The calibration curves for vitamin C, furfural, aspirin and salicylic acid were linear in the concentration ranges of 24 - 480, 0.5 - 10, 40 - 800 and 0.8 - 16 μg/ml. Their average recoveries were 100.2%, 100.3 %, 100.2% and 99.9%, with RSDs of 1.07%, 0.85%, 0.81% and 0.77%, respectively.