乙型肝炎HBV-DNA与HBs特异性免疫复合物的测定及意义

Detection and Significance of HBV-DNA and HBsAg Circulating Immune Complexes in Viral Hepatitis B

目的 :了解各型乙型肝炎 (乙肝 )中HBV DNA的含量与HBs特异性免疫复合物 (HBs IC)的关系及其意义。方法 :共检测 172例血清标本。用定量PCR测HBV DNA及ELISA法测HBs IC。结果 :(1) 各型乙肝患者HBs IC阳性率和含量都明显升高 ,但HBV DNA阳性组的乙肝患者升高更为显著 ;HBs IC与HBV DNA的测定结果无明显相关 ;(2 ) HBV DNA阳性率在不同肝病有所不同 ;在HBV DNA阳性组中 ,DNA复制量在各病型无明显差异 ;(3) .HBs IC检出率在无症状携带者最低 (59% ) ;慢性乙肝轻度、中度和原发性肝癌检出率中等 (6 0 %、75%及 80 % ) ;慢性乙肝重度 ,乙肝后肝硬化及急性乙肝检出率最高 ,均为 10 0 %。结论 :在免疫应答功能正常的机体中 ,HBV DNA的扩增常伴有HBs IC的升高。推测 :HBs IC产生主要与机体的免疫功能有关 ,对HBV

Objective:To study the relationship between HBV-DNA content and HBsAg circulating immune complexes (HBs-IC) in viral hepatitis B and its significance. Methods: ELISA and fluorescence quantitative PCR were used in the determination of HBs-IC and HBV-DNA respectively. Serum samples from 172 cases with various types of viral hepatitis B were analyzed.Results: 1.The positive rate and the level of HBs-IC were high in all hepatitis B patients,with more significant in HBV-DNA positive patients. There was no significant correlation between HBs-IC and HBV-DNA 2.The HBV-DNA positive rate was different in patients with various period of hepatitis B While in the HBV-DNA positive group,the HBV-DNA content showed no difference. 3.The HBs-IC positive rate was 59% in asymptomatic HBV carriers,60%,75% and 80% in patients with mild and moderate chronic hepatitis B and primary liver cancer respectively,and 100% in patients with severe chronic heputitis B,cirrhosis or acute hepatitis B. Conclusion:In individuals with normal immunity,the amplification of HBV-DNA is accompanied with the increase of HBs-IC. It suggests that HBs-IC plays a role in the duplication and elimination of HBV-DNA.