人胃癌移植瘤演进过程中肥大细胞的研究

Study of the Mast Cells in Xenograft of Human Gastric Carcinoma

目的 :为了探讨肥大细胞与肿瘤演进的关系。方法 :将冻存的人胃低分化粘液腺癌MGC 80 3细胞悬液先在裸小鼠皮下接种成瘤 ,瘤直径 1cm时取完整瘤组织块再分别接种到裸小鼠的皮下、胃壁上至成瘤。分早 (2 0d以内 )、中 (2 0～ 4 0d)、晚 (40d以上 )三期观察皮下和胃壁移植肿瘤的侵袭转移情况并用甲苯胺蓝染色法检测肥大细胞。结果 :皮下、胃壁移植瘤都只在晚期才出现转移 ,皮下组肺转移率 57 1%(4/ 7) ,胃壁组肺、肝、胃周淋巴结转移率皆为 50 0 %(3/ 6 ) ,并广泛扩散至肠、脾、肝、肾等处。正常皮下组织和皮下移植瘤早、中、晚三期肥大细胞数目分别为 18.77± 8.6 0 ,4 .30± 5.4 9,5.50± 3.86和 2 .6 7± 2 .2 8,正常胃壁和胃壁移植瘤早、中、晚三期肥大细胞数目分别为 12 .6 3± 4 .4 7,6 .0 3± 3.11,3.59± 1.6 9和 1.97± 2 .13,两组肥大细胞数目基本上为依次递减(P <0 .0 1)。结论 :肥大细胞在肿瘤侵袭转移过程中可能有一定的抑制作用。

Objective: To study the relationship between the mast cells and tumor development . Methods: The cell suspensions of human gastric mucous adenocarcinoma, MGC-803, were inoculated into nude mice subcutaneously. After the subcutaneous xenograft reached to 1 cm in diameter,it was removed and cut into small pieces and transplanted into the subcutis (Group A) and the stomach wall (Group B) of nude mice respectively. The biological characteristics of the xenografts were examined in three different periods (early,<20d; middle,20～40d and advanced,>40d). The mast cells were detected by toluidine blue staining. Results: The metastasis of the cancer happened in the advanced stage in both groups. The incidence of pulmonary metastasis was 57.1%(4/7) in Group A. In Group B,the incidence of metastaese to lung,liver and stomach lymph node was 50.0%(3/6) respectively. Metastases to other organs such as liver, spleen, intestine and kidneys were also found in group B. The number of mast cells in normal skin and in the xenografts of three different periods of was 18.77±8.60,4.30±5.49,5.50±3.86 and 2.67±2.28,respectively in gruop A. It was 12.63±4.47, 6.03±3.11, 3.59±1.69 and 1.97±2.13 in the normal stomach wall of and the stomach wall xenografts respectively. Conclusions: Mast cells had the suppressive function in the metastasis of tumors.