茶碱表面分子印迹聚合物微球的制备及性能

Preparation and Properties of Theophylline Surface-Imprinted Polymer Microspheres

以Poly（GMA．DVB）微球为载体，将4，4’-偶氮双（4-氰基戊酸）（ACPA）连接到微球表面，然后以茶碱为模板分子，α-甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，通过4，4＇-偶氮双（4-氰基戊酸）（ACPA）引发聚合，在Poly（GMA—DVB）微球表面上均匀地接枝分子印迹聚合物，制备茶碱表面分子印迹聚合物微球。借助红外、电镜、粒度等表征方法对微球的制备进行了评价和探讨，采用静态法研究微球对茶碱的结合性能与分子识别特性。研究表明，分子印迹微球的接枝率随着载体微球表面环氧基含量的增加以及溶剂用量的减少而增大；分子印迹微球对茶碱具有特异的识别选择性与优良的结合亲和性，相对于咖啡碱识别选择性系数为2.81～3．62，室温吸附3h内可以达到吸附平衡。

Poly （GMA-DVB） microsphere were modified with 4,4＇-azobis（4-cyanopentanoic acid）（ACPA） on the surface. Then the molecular imprinting polymer imprinted with theophylline was grafted to the surface of modified Poly （GMA-DVB） microsphere. The surface-imprinted polymer microsphere was characterized by IR, particle size analysis and scanning electron microscopy （SEM）. The adsorption and molecular recognition of theophylline by molecularly imprinted microspheres （MIMs） were measured. The influence of the synthetic conditions on the property of the material was investigated. The experimental results show that the graft ratio of MIMs increases with the increasing of the epoxy content and the reducing of the amount of solvent. MIMs show a high adsorption capacity and good recognition property, and the partition coefficient of the microspheres adsorbing theophylline is 2.81 - 3. 62. The adsorption equilibrium can be achieved within 3 h at room temperature.