滋肾益肝汤对CCl_4所致大鼠肝损伤的保护作用

Protective effects of Zishenyigandecotion on CCl_4-induced liver injury in SD rats

目的:研究滋肾益肝汤对四氯化碳(CCl4)所致SD大鼠肝损伤的影响。方法:48只SD雄性大鼠随机分为正常组、模型组、阳性对照组(联苯双酯滴丸)和滋肾益肝汤高、中、低剂量组。滋肾益肝汤低、中、高剂量组分别给予(1.0,5.0,10.0g·kg-1·d-1)的滋肾益肝汤灌胃,正常组和模型组大鼠给予1.0g·kg-1·d-1的生理盐水灌胃,阳性对照组按照联苯双酯滴丸(0.15 g·kg-1·d-1)灌胃,连续给药7 d,末次给药后,模型组、阳性对照组、滋肾益肝汤低、中、高剂量组大鼠均给予腹腔注射10%CCl4橄榄油溶液10 ml·kg-1,正常组注射10 ml·kg-1橄榄油溶液,16 h后乙醚麻醉,从腹主动脉取血,摘取肝脏、称重,比较各组大鼠肝脏组织病理学特征和肝功能指标的差异。结果:滋肾益肝汤高、中剂量组大鼠丙氨酸氨基转移酶(ALT)和门冬氨酸转氨酶(AST)活性明显低于模型组(P<0.01);肝组织中超氧化物歧化酶(SOD)活性较模型组明显升高(P<0.01);丙二醛(MDA)含量显著下降(P<0.01);病理学检查显示模型组大鼠肝细胞变性明显,并伴有大量炎细胞浸润,滋肾益肝汤高、中剂量组可使大鼠肝细胞水肿程度及炎细胞浸润程度减轻。结论:滋肾益肝汤对CCl4所致SD大鼠肝损伤具有保护作用。

OBJECTIVE To investigate the protective effects of Zishenyigan decoction on CCl4-induced liver injury in SD rats.METHODS 48 male SD rats were randomly divided into 6 groups：control（Ctrl）,model（CCl4）,positive（biphenyldim-ethylesterate pills,DDB）,and low,medium and high dose of Zishenyigan decoction（ZSYG-L,ZSYG-M and ZSYG-H） group.Rats of ZSYG-L,ZSYG-M and ZSYG-H group were given1.0,5.0,10.0g·kg-1·d-1（i.g.）Zishenyigandecoction for 7 days,respectively.While the control and model groups were given0.9% NaCl injection,respectively,the positive group was treated with DDB0.15 g·kg-1·d-1.After the last time of treatment,each group was given10% CCl4olive oil solution10 ml ·kg-1（i.p.）for 16 h except the control group with 10 ml·kg-1 olive oil solution All groups were anesthetized and the blood was sampled via abdominal aortic,the livers were resected.Liver function index,and liver issue HE staining were determined and analyzed.RESULTS Compared with the model group,ALT and AST in the serum of ZSYG-M and ZSYG-H group were significantly decreased（P0.01）.While the activation of SOD（P0.01）was increased and the level of MDA was decreased（P0.01）in liver issue.HE staining results showed that there were significantly injuries in liver cells,with a large number of inflammatory cells infiltrated in the model group. Treatment of ZSYG-M and ZSYG-H attenuated these injuries. CONCLUSION Zishenyigandecoction could protect liver of SD rats from CCl4-induced liver injury.