评价葡萄根系抗寒性指标的确定

Index for the evaluation of grape root cold-resistance

【目的】探明低温胁迫对不同葡萄品种根系抗寒性的影响。【方法】以‘康可’、‘白塞比尔’、‘公酿一号’、‘无核白’、‘葡萄园皇后’、‘龙宝’为试材,分别测定了在不同低温处理下各品种的可溶性糖、丙二醛、可溶性蛋白、脯氨酸、自由水含量的变化以及显微镜下各品种根系的解剖结构特征,并通过相关分析和主成分分析对其抗寒性进行综合评价。【结果】随着处理温度的降低,各品种根系的可溶性糖、可溶性蛋白、游离脯氨酸和丙二醛含量逐渐升高,不同品种升降的速度和幅度有明显差异;通过相关分析发现各生理生化指标与半致死温度相关性极高。各品种综合抗寒性由强到弱依次为:‘康可’,‘公酿一号’,‘白塞比尔’,‘龙宝’,‘葡萄园皇后’,‘无核白’。【结论】研究发现,半致死温度、丙二醛含量、根系解剖特征可以作为评价葡萄抗寒性的重要指标。

[Objective]The aim of the study is to understand the effect of low temperature stress on the root of different grape varieties. [Method]＇Concord＇, ＇Seibel Blanc＇, ＇Gongniang No. I＇, ＇Thompson Seed- less＇, ＇Queen of Vineyard＇ and ＇Ryo Ho＇ were used as testing materials to investigate the contents of soluble sugar, MDA, soluble protein, proline and free water under different chilling treatments. The anatomical features of the root systems were also observed under microscope. Comprehensive estimation of root cold-resistance was made through correlation analysis and principal component analysis. [Result]The contents of the soluble sugar, soluble protein, free proline and MDA in the root of all varieties gradually increased as the temperature decreased, but the increase speed and magnitude were obviously different among varieties. The physiological and biochemical indexes were highly correlated with LTs0. Comprehen- sive root cold resistances of different varieties from strong to weak were ＇ Concord＇, ＇ Gongniang No. 1＇, ＇Seibel Blanc＇, ＇Ryo Ho＇, ＇Queen of Vineyard＇ and ＇Thompson Seedless＇. [Conclusion] The contents of LT50, MDA and the anatomical features of root system could be used as important indexes for evaluating the cold hardiness of grape.