HPLC法测定不同产地牛膝中β-蜕皮甾酮的含量

Determination of β-ecdysterone in Achyranthis Bidentatae Radix from Different Areas by HPLC

目的：比较分析不同产地牛膝中β-蜕皮甾酮的含量差异，以评价其质量。方法：采用HPLC法对不同产地的牛膝f3蜕皮甾酮含量进行测定。色谱柱为Kromasil C18（250mm×4．6mm，5μm）；流动相为乙腈：水（15：85）。结果：β-蜕皮甾酮在0．2064-2．58μg范围内线性关系良好（r=0．9992），平均回收率为99．34％。结论：不同产地的牛膝中β-蜕皮甾酮含量有别，但均达到药典要求。

Objective：To determine the content of β-ecdysterone in Achyranthis Bidentatae Radix from different areas and evaluate their qualities. Methods： the content of β-ecdysterone in Achyranthis Bidentatae Radix from different areas was measured by HPLC. Kromasil C18column （250mm× 4.6mm,5μm） was used,with the mobile phase consisting of acetonitrile water（15：85）. Results： The linear range of β-ecdysterone was 0. 2064 - 2.58μg （r = 0.9992）, the average recovery rate was 99.34 %. Conclusion ： There has a difference between the content of β-ecdysterone in Achyranthis Bidentatae Radix from different areas, but meet the Pharmacopoeia requirements.