利用SPR生物传感器检测缺血修饰白蛋白

Detection of ischemia modified albumin using SPR biosensor

缺血修饰白蛋白(IMA)的检测对心肌缺血的早期诊断和治疗具有重要意义。利用纳米金颗粒和混合巯基自组装于金膜表面上,研制了一种快速检测IMA的表面等离子体共振(SPR)生物传感器。同时比较了直接法和抑制法的检测下限。结果表明:直接检测法可以检测到393ng/L的IMA,而抑制检测法检测限小于5.0 ng/L。与现有的IMA检测方法相比,SPR生物传感器具有特异性好、检测下限低以及检测耗时短等优点。

The detection of Ischemia modified albumin （IMA） is significant for early diagnosis and intervention of myocardial iscbemia patient. Present a sensitive IMA detection protocol based on surface plasmon resonance （SPR） biosensor combined nan0gold and mixed self-assembled Au film. Different assay methods are investigated and compared.Result shows that the limit of detection （LOD） is 393 ng/L for direct detection, while, as to inhibitive assay, the LOD is less than 5. 0 ng/L. Compared with other methods, SPR biosensor exhibits good repetition,in this way, the sensitivity and discrimination of the proposed biosensor are greatly improved.