摘要
目的采用血清饥饿法诱导K562细胞凋亡,比较K562细胞与人白血病骨髓间充质干细胞(LMSC)共培养前后,K562细胞凋亡的变化,并比较在常氧和低氧条件下,K562细胞凋亡情况的变化。方法通过在细胞培养体系中加入150μmol/L氯化钴(CoCl2)模拟低氧环境。采用Annexin V/碘化丙啶(PI)荧光标记法检测不同氧条件下,血清饥饿后及与LMSC共培养后K562细胞的凋亡情况。结果在单独悬浮培养组,10%胎牛血清(FBS)培养条件时,K562细胞凋亡率为(5.00±0.04)%,FBS饥饿培养(无FBS)24h后,K562细胞凋亡率为(11.40±0.63)%,较10%FBS培养明显升高(P<0.05)。常氧条件下与LMSC共培养后,K562细胞凋亡率为(7.43±0.86)%,细胞凋亡率较血清饥饿培养下降(P<0.05),在150μmol/L CoCl2存在时,细胞凋亡率下降更明显(5.91±0.35)%(P<0.05)。结论①LMSC能够抑制血清饥饿诱导的K562细胞凋亡。②在CoCl2模拟的低氧条件下,LMSC抵抗血清饥饿诱导的K562细胞凋亡的能力增强。
Objective To shed light on the effects of human leukemic bone marrow mesenchymal stem cells （LMSC） on the pathogenesis and prognosis of leukemia, we compare the apoptosis of K562 cells that cultivated with or without LMSC under normoxia or hypoxia. Methods The hypoxia environment was achieved by treating cells with 150 μmol/L cobalt chloride（CoCl2 ）. Annexin V/PI binding assay was applied to investigate apoptosis of K562 cells cultured in FBS-starvation and with LMSC. Results Apoptosis of K562 cells was significantly in- creased while treated with 0%FBS[（11. 40±0.63）%] than that treated with 10%FBS[-（5.00± 0.04）V00], but it was redecreased when they were exposed to LMSC either in n0rmoxia [ （ 7.43 ± 0.86） % ] or hypoxia [ （5.91 ±0.35） %] condition. Conclusion ① LMSC can resist FBS-starvation-induced apoptosis of K562 cells. ②The effeets of LMSC on K562 cells is enhanced under hypoxia condition.
