无花果叶中补骨脂素含量测定

Determination of Psoralen in Fig leaves

目的：建立无花果叶中补骨脂素含量的HPLC测定方法，为无花果叶的开发利用及质量标准的制定提供依据。方法：色谱柱为hypersil ODS（250mmx4．0mm、5μm）；流动相为乙腈-水（55：45）；流速为1．0mL／min；检测波长290nm；柱温32℃。结果：补骨脂素在2～10μg范围与峰面积线性关系良好（R2=0．9996），平均回收率为102．2％，RSD=1．80％。

Objective： To establish a HPLC method for the content determination of psoralen in Fig leaves. Methods： The analysisi was performed on phenomenex hydro C18 column （250mm ×4. 6mm, 4μm） , the mobile phase was Acetonitrile -H20 （55： 45） with a flow rate at 1. OmL/min, and detectional wavelength at 290nm under column temperature 32℃. Results： There was a good linear relationship when the sample size of bergenin was at a range of 2 ～ 10μg （R2 =0.9996）, the recovery was 102.2%, with RSD at 1.80%. Conclusion： This method was exact, simple, stable, fast and reproducible to be used for the content determination of psoralen in Fig leaves.