宫颈上皮内瘤变组织中P16蛋白表达与HR-HPV病毒载量的相关性研究

Correlation between P16 and HR-HPV Load in Cervical Intraepithelial Neoplasia

目的:探讨宫颈上皮内瘤变(CIN)组织中P16蛋白表达及其与高危型人乳头瘤病毒(HR-HPV)病毒载量的相关性。方法:分别采用免疫组化SP法和第二代杂交捕获法(HC-2)检测宫颈(正常宫颈42例,CINⅠ级32例,CINⅡ53例,CINⅢ92例)组织中P16蛋白表达及HR-HPV病毒载量。结果:①从正常宫颈、CINⅠ、CINⅡ到CINⅢ,宫颈组织P16蛋白表达强度及HR-HPV阳性率均递增,差异有统计学意义(P<0.01)。②不同级别CIN组织中HR-HPV病毒载量分别呈正态分布,并随病变级别增高,病毒载量中位数增高,但病毒载量与病变级别之间无相关性(r s=0.1412,P>0.05)。③P16蛋白表达强度与CIN级别呈正相关(r s=0.3633,P<0.01)。④在CIN组织中,P16蛋白表达与HR-HPV病毒载量呈正相关(r s=0.4781,P<0.01)。结论:P16、HR-HPV感染参与了CIN的发生与发展,P16免疫组化检测对宫颈癌前病变的早期诊断有很高的参考价值。

Objective：To examine the correlation between P16 and HR-HPV load in cervical intraepithelial neoplasia（CIN） and the potential clinic significance. Methods：Expression of P16 protein was detected by im- munohistochemical staining and HR-HPV load was detected by hybrid capture II （HC-2）in 177 specimens of CIN（32 cases of CIN I ,53 cases of CIN II and 92 cases of CIN III ）and 40 specimens of normal cervix. Re- sults ：The positive rates of P16 and HR-HPV in CIN were both significantly higher than those in normal cervix （P〈O. 01 ）. The HR-HPV load had a normal distribution in each level of the CIN, and the median value in each level increased with the level increasing. There was no relationship between the HR-HPV load with the CIN level（ rs = 0. 1412, P 〉 0. 05）. P16 expression in CIN increased with the CIN level increasing （ rs = 0. 3633, P 〈0. 01 ）. The expression of P16 was positively correlated to the HR-HPV load in CIN（ rs =0. 4781, P 〈0. 01 ）. Conclusions：P16 and the infection of HR-HPV contribute to development of CIN, and P16 has some value in early diagnosis of CIN.