内生条纹拟盘多毛孢发酵液中细胞毒活性成分研究

Cytotoxic secondary metabolites from the liquid culture of plant endo- phytic fungus Pestalotiopsis virgatula

从条纹拟盘多毛孢Pestalotopsis virgatula发酵液中分离得到8个化合物,其结构分别被鉴定为:2‐(1‐甲氧基‐1‐H‐吲哚‐3‐基)乙醇(1),2‐(1‐甲氧基‐1‐H‐吲哚‐3‐基)乙酸(2),3β‐羟基‐5α,8α‐过氧化麦角甾‐6,22‐二烯(3),麦角甾‐4,6,8(14),22‐四烯‐3‐酮(4),对羟基苯乙醇(5),邻苯二甲酸二异丁酯(6),(E)‐3‐(4‐羟基‐3‐甲氧苯基)败脂酸(7)和丁二酸(8),化合物1–8均为首次从该菌种中分离得到。利用MTT法测试了化合物1和2对5种人体肿瘤细胞的细胞毒活性,结果显示化合物1和2对5株肿瘤细胞株均具有一定选择性抑制活性。

Eight known compounds, 2-（1-methoxy-1H-indole-3–yl）ethanol （1）, 2-（1-methoxy-1H-indole-3-yl）acetic acid （2）, 3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene （3）, ergosta-4,6,8（14）,22-tetraen-3-one （4）, p-hydroxybenzeneethanol （5）, diisobutyl phthalate （6）, （E）-3-（4-hydroxy-3-methoxyphenyl） acrylic acid （7） and butanedioic （8）, were isolated from the fermentation broth of Pestalotiopsis virgatula, an endophytic fungus in the branch of the Kandelia obovata. The structures of 1–7 were determined on the basis of extensive analysis of NMR spectra. The structure of 8 was determined by HPLC in comparison with the authentic sample. These compounds were isolated for the first time from this fungus. Compounds 1 and 2 showed moderate selective inhibitory activities against five tumor cell lines using MTT method.