高效液相色谱法检测防脱、育发类化妆品中的非那雄胺

Determination of finasteride in anti-calvous hair cosmetics by high performance liquid chromatography

采用高效液相色谱法测定防脱、育发类化妆品中的非那雄胺，色谱条件为ZorbaxExtendC18柱（250mm×4．6mm，5μm），流动相为乙腈／10mmol·L^-1磷酸二氢钾梯度洗脱体系，流速为1．0mL·min^-1，检测波长为220nm，柱温为30℃。实验结果表明，在此条件下非那雄胺在0．8～20mg·L^-1与相应的峰面积具有良好的线性关系（R^2=0．9990），回收率为90．3％～102．0％。

A reversed - phase high performance liquid chromatography for the determination of finasteride in anti -calvous hair cosmetics was established. The sample was analyzed on a Agilent Zorbax Extend Cts column （250 mm× 4.6 mm, 5 μm） with detecting wavelength of 220 nm at 30 ℃, and eluted by mobile phase composed of acetonitrile （CH3CN） and KH2PQ （10 mmol·L^-1） with a gradient model,at a flow rate 1.0 mL min ^-1. Results showed that as the mass concentration of finasteride is in the range of 0. 8 - 20 mg·L^-1, the relationship between the mass concentration and the corresponding peak area shows good linearity （R2 = 0. 999 0）. The recoveries of finasteride achieve 90.3 % - 102. 0%.