高血压患者人巨细胞病毒感染率及其血浆中和抗体水平分析

Prevalence of human cytomegalovirus infection and analysis of its plasma neutralizing antibody level in hypertension patients

目的了解自然状态下,高血压患者群体人巨细胞病毒(human cytomegalovirus,HCMV)的感染率及血浆特异性HCMV中和抗体水平,初步探讨HCMV感染与高血压的关系。方法于2013年1月至4月在安徽省第二人民医院募集51名诊断为高血压患者的血标本,同时募集性别和年龄类似的50名体检正常者的血标本作为对照。采用间接免疫荧光染色快速微量中和试验检测血浆标本中HCMV特异性中和抗体,采用常规间接酶联免疫吸附试验(ELISA)法检测血浆HCMV特异性IgG和IgM,采用巢式PCR法检测HCMV UL93DNA。结果高血压患者组HCMV UL93DNA、HCMV IgG和IgM的阳性率分别为72.55%、70.59%和3.92%,正常对照组分别为56.00%、50.00%和2.00%,高血压患者组中HCMV DNA和HCMV IgG的阳性率高于正常对照组(P<0.05),而HCMV IgM的阳性率在两组间的差异无统计学意义。高血压患者组血浆HCMV中和抗体的平均几何滴度为38.56±20.42,正常对照组为60.12±25.38,两组差异有统计学意义(P=0.032 6)。结论高血压患者HCMV的感染率高于正常对照,但特异性中和抗体水平明显降低。提示高血压患者的HCMV体液免疫状态低下,表明高血压与HCMV感染存在相关性。

Objective To determine the prevalence of human cytomegalovirus （HCMV） infection and plasma level of its neutralizing antibody in hypertension patients, so as to discuss the association between HCMV infection and hypertension. Methods A total of 51 anti-coagulated blood samples were collected from hypertension patients and the 50 control samples were obtained from age- and sex-matched healthy controls in the Second People＇ s Hospital of Anhui Province from Jan. 2013 to Apr. 2013. Neutralizing antibody level was analyzed by rapid fluorescence micro-neutralization; plasma anti-HCMV IgG and anti-HCMV IgM were determined by enzyme-linked immuno sorbent assay （ELISA）, and HCMV UL93 DNA was detected by nested PCR. Results The positive rates of HCMV UL93 DNA and anti-HCMV IgG in hypertension patients were significantly higher than those in healthy controls （72.55%o vs 56.00% for UL93 DNA and 70. 59% vs 50. 00% for IgG, P〈0. 05）. The positive rates of anti-HCMV IgM were not significantly different between the two groups （3.92% vs 2.00%, P〉0.05）. The geometric mean titer of HCMV neutralizing antibody was 38.56 ±20.42 in hypertension patients, which was significantly lower than that in healthy controls （60. 12±25. 38, P= 0. 0326）.Conclusion HCMV positive rate is higher in hypertension patients than in healthy controls; however, the specific neutralizing antibody level of HCMV is greatly lower than that in healthy controls, suggesting that the humoral immune status is declined in hypertension patients and there is a correlation between HCMV infection and hypertension.