摘要
为了确诊疑似肉鸡和蛋鸡鼻气管鸟杆菌(0RT)感染并研究其病原的新生物学特性,对其病原进行分离鉴定并分析其16SrDNA序列特征、溶血活性、凝血活性和药物敏感性。从云南省有鼻炎症状的蛋鸡和肉鸡中分离到5株革兰氏阴性多形性杆菌,分别被命名为YNll061、YN12071、YN12072、YN12091和YN12092。生化试验表明,5株分离株氧化酶阳性,过氧化氢酶阴性,发酵葡萄糖、乳糖、半糖、麦芽糖、甘露糖和果糖,产酸不产气;16SrDNA序列分析表明,5株分离株与GenBank上12株国外代表株之间的核苷酸序列同源性为99.39/6~100%,进一步鉴定为ORT。溶血试验和凝血试验表明,5株分离株具有p溶血性,能够凝集戊二醛醛化的绵羊红细胞。5株分离株同时对链霉素、庆大霉素、卡那霉素、阿米卡星、红霉素、氧氟沙星、诺氟沙星显示多重耐药性。结果证实,云南省存在ORT感染。本研究首次报道了ORT的溶血性和凝血性,为ORT的分离鉴定和新型诊断检测方法的建立奠定了基础。
To diagnose a suspected Ornithobacterium rhinotracheale (ORT) infection and study the novel biological characterization of ORT, isolation, identification, sequence of 16 S rDNA gene, hemaggluti- nating activity,hemolytic activity and antibiotic susceptibility of ORT were studied. Five isolates of Gram- negative, polymorphic and rod-shaped bacteria, named as YNl1061, YN12071, YN12072, YN12091 and YN12092,were isolated from the layers and broilers with coryza-like respiratory disease in Yunnan Pro- vince. The 5 isolates were oxidase-positive and catalase negative,and were able to ferment glucose,lactose, galactose,maltose,mannose and fructose to produce acid but no gas. The bacteria were further identified as ORT by 16 S rDNA sequence analysis,which shared identity from 99.3% to 100% between the 5 isolates and 12 representative isolates from GenBank. Hemolytic and hemagglutinating tests showed that these 5 i- solates had/9 hemolytic activity,and were able to hemagglutinate glutaraldehyde-fixed sheep erythrocytes. Antibiotics susceptibility test showed that the isolates were of 1(50 ~ multiple antibiotic resistance to strep- tomycin, gentamicin, kanamycin amikacin, erythrocin, ofloxacin, norfloxacin. This study confirmed that the presence of ORT infection in broilers and layers in southwestern China. It is also the first report of hemag- glutinating activity and hemolytic activity of Chinese ORT isolates, and this finding will contribute to the identification and development of new detection methods for ORT.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2014年第1期55-61,共7页
Chinese Veterinary Science
基金
国家公益性行业专项资助项目(201103008)
云南省自然科学基金项目(2010ZC228)
云南省农业科技创新工程项目(2008LA019)
关键词
鼻气管鸟杆菌
分离
16
S
RDNA
溶血性
凝血性
云南省
Ornithobacterium rhinotracheale (ORT)
isolation
16 S rDNA
hemolytic activity
hemag-glutination activity
Yunnan Province