膨体聚四氟乙烯自体血管组织化提高抗菌能力的实验研究

Autologous vascularization improves antibacterial ability of expanded polytetrafluoroethylene

目的观察膨体聚四氟乙烯（expandedpolytetrafluoroethylene，ePTFE）自体血管组织化后抗菌能力的变化。方法（1）ePTFE片随机埋植在20只大鼠腹壁皮下自体血管组织化12周，观察组织长入材料情况；②每只大鼠各距背部中线2CiTI处皮下左、右分别作切口，各注入表皮葡萄球菌（SE）菌液10μL（10^5-10^8CFU／L），随机埋植ePTFE片和自体血管组织化ePTFE片，1周后取出材料，ePTFE片为对照组、自体血管组织化ePTFE片为实验组，计数表面粘附细菌量并行HE染色和扫描电镜观察。结果HE染色观察结果显示体内血管组织化ePTFE内部已有细胞浸润及组织长人，无明显炎症细胞浸润，而普通ePTFE内部可见大量炎症细胞，材料表面可观察到类似肉芽肿样组织增生。扫描电镜观察结果显示体内血管组织化ePTFE表面可见少许、分散呈簇集状生长的葡萄球菌菌落，且见血管组织覆盖材料表面，而普通ePTFE表面覆盖大面积葡萄球菌，可见大量圆形、椭圆形菌体表面突起，呈簇集状生长，材料表面未见血管组织覆盖。实验组菌落粘附数量为（4．73±0．62）×10’CFU／L，对照组菌落粘附数量为（15．46±1．33）×10^7CFU／L，术后1周实验组粘附细菌数量明显少于对照组（P〈0．05）。结论膨体聚四氟乙烯体内血管组织化后再次植入机体靶向部位，可快速与周围组织建立微循环结构，且材料表面粘附细菌数量减少，从而提高膨体聚四氟乙烯材料的抗菌能力。

Objective To evaluate the antibacterial ability of expanded polytetrafluoroethylene （ePTFE） after autologous vascularization in rats. rats subcutaneously for autologous vascularization Methods ePTFE films were randomly implanted into 20 for 12 weeks. The tissue growth in the materials was observed. Incisions were made on the two sides （distance = 2 cm ） of the back midline. Staphylococcus epidermidis of 10 μL （ 105 - 10^6 CFU/L） was injected into the incisions, and ePTFE with or without autologous vascularization were implanted into the incisions. After 1 week, the materials were taken out to count the number of adhered bacteria. Results HE staining results displayed that eF＇TFE after autologous vasculariza- tion had normal cell infiltration and tissue growth but no obvious inflammatory cell infiltration, while ePTFE without autologous vascularization had a large amount of inflammatory cells. Scanning electron microscopy showed blood vessel tissue and a few of bacterial colonies on the surface of ePTFE after autologous vasculariza- tion, but no blood vessel tissue and a large amount of bacterial colonies on the surface of ePTFE without autologous vascularization. The number of adhesion colonies was （4.73 ± 0.62） x 107CFU/L in the autologous vascularization group and （15.46 ±1.33 ） x 107 CFU/L in the control group which was significantly less （P 〈 0.05 ）. Conclusion Microcirculation can be quickly established between ePTFE implants after autologous vascularization and the surrounding tissues, eFI＇FE implants after autologous vascularization show reduced bacteria adhesion and imoroved the antibacterial ability.