三七总皂甙对大鼠脑出血灶周围神经元线粒体超微结构的影响

Effects of total panax notoginseng saponins on ultrastructure of mitochondria for intracerebral hemorrhage focus in rats

目的:研究三七总皂甙对大鼠脑出血后神经元线粒体超微结构的保护作用。方法:用鼠脑立体定位仪将肝素化Ⅶ型胶原酶注入实验大鼠基底节区制备大鼠脑出血模型;用透射电镜观察线粒体超微结构并对其进行体视学分析;用免疫组织化学方法检测Bcl-2和Bax蛋白表达;利用神经功能评分标准检测各组大鼠的神经行为学变化。结果:神经行为学检测,模型组大鼠的神经功能评分分值升高,治疗组大鼠分值较模型组下降(P<0.01);线粒体体视学分析,治疗组线粒体的比表面积、比膜面积、体密度、数密度均较模型组增大,差异有统计学意义(P<0.01);与模型组比较,治疗组Bax的阳性表达降低(P<0.01)、Bcl-2的阳性表达升高(P<0.01),Bcl-2/Bax蛋白比值增高。结论:三七总皂甙能够保护脑出血后神经元线粒体,促进神经功能恢复。

Objective： To study the protection of total panax notoginseng saponins on neurons adjacent to intracerebral hemorrhage focus（ICH） in the rats. Methods： ICH model was established by injecting collagenase Ⅶ into the brain of rats. The uhrastructure of neurons that surround intraeerebral hemorrhage area in rats was observed by transmission elec- tron microscope and the mitoehondria were analyzed with stereology method. Immunohistoehemical staining technique was used to demonstrate expressions of Bcl-2 and Bax proteins in penumbra area. Berderson score was used to evaluate behav- ioral change. Results： Stereology analysis revealed that compared with model group, the volume density, numerical den- sity,specific surface and specific membrane of the neuronal mitochondria, increased notably in total panax notoginseng saponins treatmented group （ P 〈 0.05 ）. The result of immuno- histoehemistry showed that the expression of Bcl-2 in treatment group is higher than that in model group （P 〈 0.01 ） ;However the expression of Bax degraded prominently and the ratio of Bcl-2/Bax increased in treatment group. The score of behavioral function was obviously increased after ICH in rats, indicating an obvious neurologic impairment. Conclusion：Total panax notoginseng saponins could protect neurons located near the hemorrhage brain area.