黄芩提取化合物SBX对NSCLC细胞系细胞增殖及信号传导通路影响研究

Influence of Skutellaria extract SBX on NSCLC cell proliferation and signaling networks

目的:观察黄芩提取化合物单体成分SBX对人NSCLC细胞系H1975和H1944细胞株增殖的影响,初步探讨SBX对NSCLC细胞系抑制的机制。方法:体外培养人H1975和H1944细胞株,以0、10、25、50、100和200μmol/L的SBX处理细胞,应用MTT法检测SBX对细胞增殖的影响,筛选出敏感细胞株。应用流式细胞术检测SBX对敏感细胞株细胞周期的影响,应用Protein Pathway Array技术检测SBX对敏感细胞株蛋白表达的影响。结果:SBX对两种类型的NSCLC细胞株均有抑制作用,并呈剂量依赖性,对H1975和H1944细胞株的IC50值分别为(38.3±1.25)和(62.5±1.76)μmol/L。SBX对H1975和H1944细胞抑制作用比较,对H1975细胞抑制作用更强,P<0.001。流式细胞术显示,SBX对H1975细胞周期的影响主要是引起G0/G1期阻滞和凋亡增加,与空白对照组相比,P<0.001。50和100μg/mL的SBX分别作用于H1975细胞后,阻滞在G0/G1期的细胞由35.47%分别升至54.36%和73.37%,S期的细胞由61.60%分别降至41.63%和19.14%。应用Protein Pathway Array技术分析SBX对H1975蛋白表达的影响显示,共检测166个磷酸化和非磷酸化蛋白,其中53个蛋白表达,有差异性表达的蛋白共15个。其中Cdk6、EGFR、Survivin和mTor蛋白表达水平上调,而p27、p-RB、Cyclin D1、Cyclin E、XIAP、p53、p-p53、p-AKT、Akt、Notch4和Cdk4蛋白表达水平下调。结论:SBX能够抑制人NSCLC细胞系H1975和H1944细胞株的增殖,敏感细胞株为H1975;SBX主要影响H1975的EGFR和Notch信号传导通路,并通过以上信号传导通路影响细胞周期相关蛋白表达水平的变化,导致细胞周期G0/G1期阻滞,凋亡增加。

OBJECTIVE：To investigate influence of Skutellaria extract SBX on the proliferation of NSCLC cell lines H1975,H1944,and preliminarily explore the mechanism of SBX inhibition on NSCLC cell lines. METHEODS： The cell lines（H1975, H1944） were in vitro cultured and its growth inhibition was detected by MTT. After screening the most sensitive cell line,the effects of SBX on cell cycle was analyzed by flow cytometry and the protein expressions determined by Protein Pathway Array. RESULTS： SBX could significantly inhibit the proliferation of the two NSCLC cell lines in a dose-dependent manner,and ICs0 value on H1975, H1944 were （38.3--＋_ 1.25） and （62.5 ~ 1.76） t^mol/L respectively. Compared with H1944, H 1975 was more sensitive to SBX （P〈0.001 ）. Flow cytometric analysis showed that SBX arres- ted H1975 cells in the G0/GI phase with concurrent increase of apoptosis（compared with control group,P〈0. 001）. The percentages of cell arrest at Go/G1 phase increased from 35.47 % in control cells to 54.36 % and 73.37 ~, the percentages of cell at S phase decreased from 61.60G in control cells to 41.63% and 19. 14% ,in ceils treated with 50 μg/mL and 100μg/mL of SBX respectively. The Pathway Array analysis,using a set of 166 protein- and phospho-specific antibodies, revealed that a total 53 proteins were expressed, of those 15 were differentially expressed. The assessment indicatedprotein expression of Cdk6 ,EGFR,Survivin and mTor of H1975 cells increased after treatment with SBX,and p27 ,p-RB, Cyclin D1 ,Cyclin E, XIAP, p53, p-p53, p-AKT, Akt, Notch4 and Cdk4 was decreased. CONCLUSIONS： SBX can inhibit the proliferation of two human NSCLC cell lines and H1975 is a sensitive cell line. SBX significantly affects EGFR and Notch signal network,through which affects the expression of the cell cycle proteins and results in G0/G1 arrest and in- creases apoptosis in H1975 cells.