红花中羟基红花黄色素A的提取纯化工艺研究

Extraction and Purification Technology of Hydroxysafflor Yellow A from Carthamus Tinctorius

目的:优化红花中羟基红花黄色素A(HSYA)的提取纯化工艺。方法:以羟基红花黄色素A的提取率为考核指标,采用正交试验设计对红花提取工艺参数进行考察,进而考察大孔树脂对羟基红花黄色素A的纯化。结果:红花提取工艺条件是20倍量水浸泡40min;再采用HPD100大孔树脂对羟基红花黄色素A纯化,得到纯度较高的HSYA。结论:优选羟基红花黄色素A的提取纯化工艺操作简单、科学合理,可用于其单体及制剂的制备。

Objective ： To optimize the extraction and purification process of hydroxysafflor yellow A （HSYA） from Cartha- mus tinctorius. Method ： Take the content of HSYA as assessment index and use orthogonal design to optimize the extrac- tion parameter of Carthamus tinctorius, and purify the HSYA by the varied type models of macroporous resin. Result： The optimum extraction condition was 20 times the volume of water soaking 40min, And the high purity HSYA could be obtained by purifying from HPD100 models of macroporous resin. Conclusion：The optimum extraction and purification technology is reasonable and scientific, and it can be used for large scale production.