茚三酮柱后衍生氨基酸分析仪测定卷烟主流烟气中的氨

Determination of Ammonia in Mainstream Cigarette Smoke by Amino Acid Analyzer with Ninhydrin Post-column Derivatization

为了避免离子色谱分析氨时常见干扰离子钠对检测准确性的影响，首次利用氨与茚三酮显色反应的选择专一性，建立了使用氨基酸分析仪测定卷烟主流烟气中氨的分析方法。采用烟草行业氨检测标准（YC／T377—2010）对烟气样品进行前处理，使用SYKAMLCAK06／Na（4．6×150mm）离子色谱柱，钠系统缓冲液体系，通过优化氨基酸分析仪色谱分析条件，实现了氨的自动柱后衍生分离分析。结果表明，氨的质量浓度范围为0．1001-1．2019μg/mL峰面积与质量浓度具有良好的线性关系，相关系数为0．99958，方法的检出限0．142μg/支，RSD％为1．25％，平均回收率为97．6％。该方法不存在钠离子干扰问题，适用于卷烟主流烟气中氨的准确测定。

In order to avoid the detrimental effect of Na＋ to the determination of ammonia by ion chromatography, a new method was established and applied for ammonia determination in mainstream cigarette smoke by post-column derivatization with ninhydrin by using amino acid analyzer. The pretreatment for tobacco smoke samples was carried out according to the industrial standard method （YC/T 377-2010）. The chromatographic conditions were optimized to achieve separation and post-column derivatization of ammonia by using SYKAM LCAK06/Na （4.6x 150 mm） ion chromatogram column and Na＋ buffer system. The results showed that the linear range of the proposed method was 0.1001-1.2019μg/mL, the detection limit was 0.145 μg/cig, with the RSD% and the average recovery rate were 1.25% and 97.6%, respectively. This method has a distinct advantage in interference of Na＋ and is adequate for quick and sensitive determination of ammonia in mainstream cigarette smoke.