产蛋白酶和淀粉酶菌株的筛选及其发酵产酶特性研究

Screening of Protease and α-amylase Producing Strains and Its Fermentation Properties

利用酪蛋白固体平板分离培养基、淀粉平板分离固体培养基初筛,液体发酵产酶培养基摇瓶复筛相结合,从实验室保藏的真菌菌株中筛选出一株产蛋白酶的真菌菌株和一株产淀粉酶的真菌菌株,对其固态发酵特性进行了研究.结果表明:蛋白酶产酶高峰为24 h,酶活力达到3760 U/g;淀粉酶产酶高峰为32 h,酶活力达到0.405 U/g.

The strains Aspergillus niger with higher protease production,Aspergillus oryzae with higher a-amylase production were screened from fungal strains in laboratory,through the preliminary screening with Casein plate and Soluble starch plate solid culture medium, the second screening with liquid fermentation enzyme production medium of shake flash fermentation. It was studied on producing enzymes by solid-state fermention. The results showed that the protease activity was 3760 U/g for fermentating 24 h, while the fungal c^-amylase activity was 0.405 U/g for fermentating 32 h.