奶牛乳房炎葡萄球菌的分离鉴定及耐药性分析

Isolation and identification and drug resistance analysis of staphylococcu causing dairy cow mastitis

试验采集了临床型乳房炎奶牛的乳汁进行细菌分离鉴定,并用14种抗生素进行药物敏感性检测,以确定分离株耐药谱.结果表明:分离出的85株葡萄球菌,其中42株耐药葡萄球菌,检出率为49.4%.葡萄球菌对青霉素、阿莫西林、红霉素、卡那霉素、庆大霉素、磺胺类药物、四环素、头孢唑林、苯唑西林的耐药率分别为73.8%、69%、61.9%、58%、19%、26.2%、7.1%、16.7%和28.6%,而对利福平、氯霉素、万古霉素、大观霉素、环丙沙星耐药率都为0.说明耐药性葡萄球菌检出率比较高,耐药性严重,并呈多重耐药性,而利福平、氯霉素、万古霉素、大观霉素、怀丙沙星有较好的抗菌活性.

Milk of clinical cow with mastitis was used for bacteria isolation and identification,and its drug sensitivity to 14 antibiotics was detected to determine the drug resistance spectrum of the isolated strains.The result showed that for all the isolated 85 staphylococci,42 were drug resistant,with a positive rate at 49.4%.The resistance rate for the 42 strains to penicillin,amoxicillin,erythromycin,kanamycin, gentamicin,sulfonamides,tetracycline,cefazolin and oxacillin were 73.8%,69%,61.9%,58%,19%,26. 2%,7.1%,16.7% and 28.6%,respectively,while to rifampicin,chloramphenicol,vancomycin,spectinomy-cin and ciprofloxacin hydrochloride were all 0,indicating that staphylococci were with relatively higher and multiple drug resistance,while rifampicin,chloramphenicol,vancomycin,spectinomycin and ciprofloxacin hydrochloride were with better anti-bacteria activity,which provides experimental basis for mastitis protec-tion of cow.