三疣梭子蟹的两种标记技术

Two types of tagging techniques for Portunus trituberculatus

本研究采用注射可视嵌入性荧光、剪附肢两种手段对不同期别的三疣梭子蟹进行标记,以研究标记的适用性及对个体生长的影响。对不同发育阶段三疣梭子蟹进行两个部位荧光注射,统计蜕壳后可识别率。结果显示,Ⅱ、Ⅲ期幼蟹适合腹面区域注射,Ⅳ期以后幼蟹适合游泳足基节和头胸甲背面的薄膜关节注射;标记后,经过两次蜕壳,识别率在80%以上,但经3次蜕壳后,识别率较低;根据标记组、未标记组生长数据,通过单因素方差分析发现,标记对三疣梭子蟹个体生长无显著性影响。Ⅶ期以后的幼蟹,更适合剪附肢法,该法操作简单、识别率高,对个体生长、存活无显著性影响(P>0.05)。

The suitability of visible implant fluorescent elastomer （VIE） and shear ap pendage for tagging Portunus trituberculatus was investigated. VIE was implanted into two body positions of P. trituberculatus at different stage. Feasibility was evaluated based on tag retention, the impact of tagging site on growth and survival, and juvenile size. We found that VIE injection in the ventral region is suitable for Stage Ⅱ and Ⅲ juvenile crabs. The joint of swimming leg coax and carapace are suitable for Stage IV and later stage juvenile crabs. Tag re tention rate was more than 80% for crabs that molted twice, but it was lower for crabs that molted three times. The growth and survival rates were compared with those of an equal num ber of untagged crabs. The results showed that tags had no impact on the growth or survival of juvenile crabs. Due to the higher tag retention rate and simplicity of operation, shear appendage tagging is recommended for Stage Ⅶ juvenile crabs and the older. The two tagging techniques can be used for select-breeding and enhancement of crab.