高效液相色谱法测定卤虫无节幼体中精胺含量

Determination of spermine in brine shrimp by HPLC

本研究建立了高效液相色谱法测定卤虫无节幼体中精胺含量的方法。样品经5％三氯乙酸溶液提取，提取液采用丹璜酰氯柱前衍生，以甲醇一水为流动相，经C18反相色谱柱分离后用紫外检测器检测。精胺的线性范围为0．1～1000μg/ml，线性相关系数为0．9999，检出限为0．06μg/ml。3个添加浓度（0。1、1．0和5.0μg/m1）的平均回收率为86．3％～96.39/6，相对标准偏差皆小于59/6。采用此方法对卤虫中精胺进行检测，其精胺含量为0．077mg/g。本方法具有分析时间短、线性范围宽、灵敏度和精密度高等优点，适合于卤虫中精胺的检测．

An analytical method was established for the determination of spermine in brine shrimp by high performance liquid chromatography （HPLC） with ultraviolet detector. Spermine in brine shrimp samples was extracted with a mixture of 5% trichloroacetic acid, then derived with dansyl chloride, followed by separation via the C18 RP-HPLC column with methanol-wa- ter. Under the optimal conditions, spermine showed good linear relationship in the range of 0.1-1000 gg/ml and the correlation coefficient was 0. 999 9. At the spiked levels of 0.1, 1.0 and 5.0μg/ml, the average recovery was 86.3 %-96.3 % with the relative standard deviation less than 5 %. The minimum detection concentration was 0.06Mg/ml. The overall process was suc cessfully applied to identify and quantify spermine in brine shrimp, and the value was 0. 077mg/ g. The method is sensitive, rapid and precise.