花生中蔗糖合成酶基因AhSuSy在花生中的组织表达及对非生物胁迫响应研究

Expression Analysis of the Sucrose Synthase Gene AhSuSyin Different Tissues and under Abiotic Stresses in Peanut

低温、高盐和干旱胁迫严重影响植物的生长和产量。本研究以花生品种花育33号为实验材料,根据cDNA文库中已知的蔗糖合成酶基因AhSuSy全长序列设计引物,通过RT-PCR克隆到该基因。通过荧光定量PCR分析了该基因在花生各组织中的表达及在低温、高盐等非生物胁迫下的表达。结果显示,该基因为组成型表达基因,在叶片和根中表达量较高,在花中表达量最低;AhSuSy基因在花生的叶片和根中对低温均没有明显响应,但在花生根中受高盐胁迫和干旱胁迫明显诱导,说明该基因可能参与了花生对高盐和干旱胁迫的适应性调控;AhSuSy在花生根中受ABA的明显诱导,说明该基因对花生非生物胁迫的调控可能是以依赖ABA的方式进行的。

Plant growth and yield are strongly influenced by abiotic stresses such as drought, salt and cold. In this article, we cloned the full length of sucrose synthase gene AhSuSy from peanut （Arachis hypogaea L. cultivar Huayu33） through RT-PCR method. The expression pattern of Ah- SuSy in different peanut tissues and under various abiotic stresses were detected with realtime RT- PCR. The results showed that AhSuSy was expressed in all the six tissues studied, with higher expression level in the leaves and roots and the lowest level in the flowers. The expression of AhSuSy had no obvious change under cold stress in peanut leaves and roots. However, AhSuSy was induced distinctly in peanut roots during salt and drought conditions. These results suggested that AhSuSy may participate in the salt and drought stress regulation o{ peanut. The expression of AhSuSy was also induced by exogenous ABA, which indicated that AhSuSy may regulate peanut abiotic stress resist ance through ABA-dependent way.