五味子乙素对巨噬细胞炎症相关介质表达的影响

Effect of Schisandrin B on inflammatory related medium expression of macrophage

目的探讨五味子乙素(Schisandrin B)的抗炎作用及其机制,为指导治疗妇科炎症提供理论依据。方法分离和培养小鼠腹腔内的巨噬细胞,随机分为Normal组(DMSO)、DEX组(地塞米松1×10-7 mmol/L)、LPS组(1.1×10-2 mmol/L)和Schisandrin+LPS组,Schisandrin+LPS组根据给剂量分为4个亚组(4×10-5 mmol/L、4×10-6mmol/L、4×10-7 mmol/L、4×10-8 mmol/L)。利用ELISA法检测培基上清中IL-6、TNF-α、NO的含量,RT-PCR法检测巨噬细胞炎症模型中IL-1β的表达。结果 Schisandrin+LPS组和DEX组的巨噬细胞IL-1β的表达量明显低于Normal组(P<0.05),且Schisandrin+LPS组的巨噬细胞IL-1β的表达低于DEX组(P<0.05);Schisandrin+LPS组的上清液中TNF-α、IL-6及NO含量低于Normal组且呈剂量依赖性。结论 Schisandrin B可通过对TNF-α、IL-1β、IL-6、NO表达的抑制来实现抗炎作用。

Objective To investigate Schisandrin B the anti-inflammatory effect and its mechanism, and provide a theoretical basis for guiding the treatment of gynecological inflammation. Methods The mouse peritoneal macrophages were isolated and cultured, which were divided randomly into 4 groups：Normal Group,DEX group （Dexamethasone 1 X 10 7 mmol/ L）, LPS Group （1.1）〈-10-2 mmol/L） and Schisandrin＋ LPS Group. Sehisandrin＋ LPS Group was divided into 4 subgroup by dose of Schisandrin （4 X 10.5 mmol/L, 4 X 10-6 mmol/L, 4 X 10 7 mmol/L, 4 X 10-8 mmol/L）. The contents of IL-6 ,TNF-a,and NO were detected with ELISA assay. The expression of IL-13 was detected by RT-PCR. Results In macrophage inflammatory model the IL-I expression of Schisandrin＋ LPS groups and DEX group were less than that of Normal group （P0.05） ,and the IL-I expression of Schisandrin＋LPS groups were less than that of DEX group（P0.05）. The TNF-,IL-6 and NO of the supernatant in Schisandrin＋LPS groups were less than that of Normal group. Schisandrin B reduced the amount of TNF-, IL-6 and NO, which was dose-dependent. Conclusion Schisandrin B exerts anti-neuroinflammatory activity by inhibiting the expression of TNF-a,IL-I,IL-6 and NO.