鸡白痢沙门菌的分离鉴定、耐药性及分子分型研究

Isolation and Identification of Salmonella pullorum,Drug Sensitivity and Molecular Typing

为了解广东和广西两省鸡白痢沙门菌的耐药现状及其分子流行病学信息,对疑似鸡白痢沙门菌进行了病原菌分离鉴定.根据鸡白痢沙门菌invA基因进行PCR扩增鉴定,应用PFGE方法对菌株进行分型研究、采用纸片法检测菌株对18种抗菌药的药物敏感性.结果表明,分离到40株鸡白痢沙门菌;PFGE分型结果显示可分为22个不同型别,其相似值约为62％～100％,聚类分析和流行病学发现,广东和广西两省的11个不同地区间存在同源菌株,同个地区也有同源菌株;菌株对青霉素G、利福平、红霉素、等药物等常用药物高度耐药.临床治疗建议避免使用青霉素G、利福平和红霉素,应重视鸡白痢沙门菌的耐药情况;鸡白痢的流行与种鸡感染具有相关性,在防控该病时应加强种鸡的防疫措施.

To understand the drug resistance and molecular epidemiological of Salmonella pullorum in Guangdong and Guangxi provinces,The suspected materials were identified by PCR. PFGE was applied to analyze the molecular epidemiology of the strains, the antimicrobial susceptility was determined by disk diffusion method. PFGE showed 40 strains were divided into 22 clusters, the similarity rate is 62-100%, clustering analysis and epidemiology found that 11 different areas in Guanggong and Guangxi homologous strains. The isolates are high sensitive to penicillin G, rifampicin and erythromycin. The clinical treatment should avoid the use of penicillin G, rifampicin and erythromycin. We should pay more attention in antimi- crobial resistance and strengthening feeding management in preventing and controlling the disease.