摘要
试验从健康猪(非哺乳期仔猪)、鸡及传统乳制品中分离到30株乳酸菌,镜检、生化鉴定后对其中的6株高性能菌株进行耐酸耐胆盐试验。结果显示,所分得的30株乳酸菌中18株为乳杆菌,其余12株为乳球菌;LS和LJ2菌株在pH 2.0环境下的存活率分别为135%和124%;LZ1株在pH 3.0环境下的存活率为137%;LJ1株对猪胆盐有较高耐受能力,LT株对牛胆盐有较高耐受能力。这些高生存性菌株的获得有助于进一步研究乳酸菌的益生特性,也可以作为功能性菌株深入研究。
30 strains of Lactobacillus were isolated from intestines of pigs and chickens, and traditional fermented milk products. After microscopy and biochemistry identification, 6 strains were used for acid and bile salt tolerance tests. The results showed that among 30 strains, 18 strains were Lactobacillus, others were Lactococcus. LS and LJ2 have a high survival rate for 135%and 124 under pH2. 0 growth environment, LZ1 can tolerate pH3.0 growth environment, its survival rate is 137 ; LJ1 has a higher tolerance to bile salt, LT has a higher tolerance to bovine bile salt. These high survivability strains can help to further study on the probiotic properties and functions of Lactobacillus .
出处
《动物医学进展》
CSCD
北大核心
2014年第2期73-77,共5页
Progress In Veterinary Medicine
基金
内蒙古自然科学基金项目(31160508)
关键词
乳酸菌
高生存性
耐酸
耐胆盐
Lactobacillus
high survivability
acid resistance
bile salt tolerance
