高活性抗氧化肽的体外胃肠耐受性研究

Tolerance of highly active antioxidant peptides during in vitro gastrointestinal

研究了高活性抗氧化肽在体外模拟胃肠消化吸收过程中的耐受性。以6条抗氧化合成肽为研究对象,采用体外胃肠消化模型和Caco-2细胞单层模型,模拟其胃肠消化吸收过程,分别测定抗氧化肽在胃肠消化中的降解与转化、含量变化及以TEAC活性和ORAC活性为指标的抗氧化活性变化。结果表明,6条抗氧化肽均耐受胃消化,不耐受肠的消化,但是经胃肠消化吸收后仍具有很强的抗氧化活性,分别高于阳性对照还原型GSH和Vc;YPWY、GWEW和YEW在肠阶段发生C端氨基酸降解,生成短肽YPW、GWE和YE,而且可以完整地透过Caco-2细胞。这项研究为这些抗氧化肽作为功能配料进行开发利用提供了理论依据。

The tolerance of highly active antioxidant peptides during in vitro simulated gastrointestinal digestion were researched. The object of this study were six antioxidant peptides. In vitro gastrointestinal digestion model and Caco-2 cell monolayer model were used to simulated the process of their gastrointestinal digestion. And determined the degradation and transform, change of concentration and change of antioxidative acrivity which used TEAC activity and ORAC activity as the index of antioxidative peptides during gastrointestinal digestion. The results showed that six antioxidative peptides could tolerate the gastric digestion, but could not tolerate the intestinal digestion, while after gastrointestinal digestion and absorption, they possessed high antioxidant activity which higher than those of positive control reduced GSH and Vc; the amino acids at C terminus of YPWY, GWEW and YEW were cut down, and respectively produced oligopeptides YPW, GWE and YE, which could be transport intact across Caco-2 cell monolayer. This study provided theoretical evidence for conducting development and ultilization of antioxidant peptides as functional food additives.