生鲜蔬菜中阪崎克罗诺杆菌的分离与鉴定

Isolation and identification of Cronobacter sakazakii from raw vegetables

从上海市售蔬菜中共采集样品102份,按照国标方法GB 4789.40—2010进行阪崎克罗诺杆菌的分离与鉴定。采用生化实验、PCR检测方法、BAX System Q7全自动病原微生物检测仪、API 20E试剂条鉴定和16S rDNA序列的测定对分离到的可疑菌株进行鉴定与确证。根据国标法的鉴定结果,102份样品中共有18份样品分离到可疑阪崎克罗诺杆菌菌株,进一步结合其他4种鉴定方法确定13份样品中分离到的可疑菌株为阪崎克罗诺杆菌菌株,分离率达13%(13/102)。通过研究说明生鲜蔬菜也是阪崎克罗诺杆菌比较常见的宿主或者污染源之一。

Cronobacter sakazakii was isolated from 102 samples of vegetables at the retail markets in Shanghai city based on national standard method of GB 4789.40—2010. Cronobacter sakazakii isolates were identified by physiological and biochemical detection, PCR method, BAX System automatic pathogenic microorganism detection system, API 20E identification, and 16S rDNA sequencing. 18 of 102 samples were isolated and identified as suspicious Cronobacter sakazakii isolates according to national standard method of GB 4789.40—2010. Strains from 13 samples were finally identified to be Cronobacter sakazakii with a separation rate of 13%, when isolates from 18 samples were tested by further other four detecting methods. It has been confirmed that vegetables are one of the most common habitats or sources of contamination in current study.