摘要
目的建立测定保婴镇惊丸中甘草苷含量的高效液相色谱法。方法采用十八烷基硅烷键合硅胶柱,AgilentC,8(250mm×4.6mm,5μm),流动相为乙腈一0.2%磷酸溶液(14:86),检测波长为276nm,柱温为40℃,流速1.0mL/min。结果甘草苷进样量在0.0973~0.9730μg范围内与峰面积呈良好线性关系(r=0.9996);平均回收率为98.01%,RSD=1.08%(n:6)。结论该方法操作简便、准确性好,可作为保婴镇惊丸中甘草苷的质量控制方法。
Objective To establish a method for the determination of the liquirtin content in Baoyingzhenjing Pill. Methods The Sym- metry C,s column(250 mm4.6 ram,5 m) was adopted with acetonitrile-0.2% phosphoric acid(14:86) as the mobile phase at a flow rate of 1.0 mL/min,the temperature was 40 and the detection wavelength was 276 nm. Results The linear range of liquirtin was 0.097 3-0.973 0 g (r=0.999 6) and the average recovery rate was 98.01% ,RSD= 1.08% (n=6).Conclusion This method is simple to operate,highly accurate and applicable for the quality control of liquirtin of Baoyingzhenjing Pill.
出处
《中国药业》
CAS
2014年第2期36-37,共2页
China Pharmaceuticals