实时定量PCR检测口腔组织中CD44 mRNA的表达

Detection the expression of CD44 mRNA in Oral Tissues by Real-Time PCR

目的:研究细胞黏附分子CD44mRNA在口腔组织中的表达情况,探讨CD44与口腔鳞癌之间的关系。方法:应用实时定量PCR(real-time PCR,RT-PCR)检测17例正常口腔组织及14例口腔鳞癌组织中CD44mRNA的表达情况。结果:CD44 mRNA在口腔鳞癌组织中的表达显著低于正常口腔组织中的表达(P=0.038);与正常口腔组织中CD44mRNA的表达水平相比较,其中有12例(86%)口腔鳞癌组织表达下调,2例(14%)口腔鳞癌组织表达上调。结论:口腔鳞癌组织中CD44mRNA的表达显著低于正常口腔组织中的表达,因此CD44的表达量可能与口腔鳞癌的发病及转移有关。

Objective：In order to study the relationship between CD44 and oral squamous cell carcinomas, in this paper we discuss the expression of CD44 mRNA in oral tissues. Methods： Detected the expression level of CD44 mRNA in 17 cases of normal oral tissues and 14 cases of oral squamous carcinoma tissue by real-time PCR. Results： The expression level of CD44 mRNA in oral squamous carcinoma tissues was significantly lower than in normal oral tissues （P=0.038）;Compared with the expression level of CD44 mRNA in nomlal oral tissues,There had 12 cases （86%） decreased but had 2 cases increased in oral squamous carcinoma tissues. Conclusion： The expression level of CD44 mRNA in oral squamous carcinoma tissues was significantly lower than in normal oral tissues, So the expression level of CD44 may be associated with the morbidity and metastasis of oral squamous cell carcinomas.