芸薹类蔬菜基因组DNA遗传多样性的RAPD分析

Analysis of genetic polymorphisms in vegetable crops of Brassica campestris by RAPD markers

采用随机引物扩增多态性 DNA( RAPD)技术 ,对芸薹类 ( 2 n=2 0 )蔬菜作物的 3 3个品种进行了遗传多样性检测 .从 70个引物中筛选出 3 7个引物 ,共扩增出 3 53带 .其中 ,多态带有 2 60条 ,扩增片段长度大多数集中在 0 .9～ 1 .6kb之间 .不同引物的检测效率相差很大 .运用 5个引物扩增的 1 0条RAPD特征带 ,可以作为一组 DNA指纹 ,区分所有供试

WT5”BZ]The genetic polymorphisms in vegetable crops of Brassica campestris was investigated using random amplified polymorphic DNA markers(RAPDs), the detected materials included thirty three cultivars. Thirty seven primers selected from seventy primers amplified 353 RAPD fragments and 260 bands of them showed polymorphisms. The length of most amplified fragments ranged from 0.9 to 1.6 kb. Different primers exhibited different detective efficiency. There were ten RAPD characteristic markers , which were detected as a group of DNA fingerprints to identify all the seven cultivars of heading type Chinese cabbage. [WT5”HZ]