雾化吸入三氧化二砷及艾叶油对哮喘豚鼠白三烯C4及Th1/Th2失衡的影响

Effect of inhaled arsenic trioxide and moxa leaf oil on leukotriene C4 and Th1/Th2 imbalance in asthmatic guinea pigs

目的 探讨三氧化二砷（As2O3）及艾叶油雾化吸入对哮喘豚鼠白三烯C4及Th1/Th2失衡的影响及平喘的机制.方法 采用卵白蛋白（OVA）致敏激发复制哮喘豚鼠模型,60只豚鼠随机分为As2O3雾化吸入组[B1：2.0 mg/（kg·d）、B2：4.0 mg/（kg·d）]、联合用药组[C1：2.0 mg/（kg·d）＋ 0.05 ml、C2：4.0mg/（kg·d）＋0.1 ml];同时设生理盐水雾化吸入组（A组）及正常对照组（D组）.用药7天后,比较支气管肺泡灌洗液（BALF）中白三烯C4的变化及Th 1/Th2失衡的情况.结果 （1）LTC4：B1、B2、C1、C2与A、D组比较差异均有统计学意义（P＜0.01）;B1与C1组比较差异有统计学意义（P＜ 0.01）; B2与C2组比较差异有统计学意义P（＜0.05）;B1与B2组比较差异无统计学意义（P＞0.05）.（2） Th1/Th2失衡：①IFN-γ：B1、B2、C1、C2与A、D组比较差异均有统计学意义（P＜0.01）,用药四组之间比较差异无统计学意义（P＞0.05）;②IL-4：B1与B2组比较差异有统计学意义（P＜0.01）,B1与C1组、B2与C2组比较差异无统计学意义（P＞0.05）;③IFN-γ/IL-4：B1与B2组比较差异有统计学意义（P＜0.05）,B1与C1组、B2与C2组比较差异无统计学意义（P＞0.05）.结论 白三烯C4的升高及Th1/Th2的失衡是支气管哮喘的重要发病机制,As2O3平喘的机制与降低LTC4及调节Th 1/Th2的失衡有关;艾叶油平喘的机制与降低LTC4有关,较小剂量的As2O3及艾叶油联合雾化吸入可安全有效地达到平喘的作用,二者联合应用有协同作用.

Objective To investigate the effects of leukotriene C4 （LTC4） and Th1/Th2 imbalance in asthmatic guinea pigs and explore the mechanism of antiasthma by inhaled arsenic trioxide （As2O3） and moxa leaf oil.Methods The guinea pig asthma models were established with ovalbumin （OVA） challenge method.60 guinea pigs wen randomly divided into As2O3 atomization inhalation group （B1：4.0 mg/kg/d,B2：4.0 mg/kg/d）,combined treatment group （C1：2.0 mg/kg/d ＋ 0.05 ml,C2：2.0 mg/kg/d＋0.1 ml）,saline aerosol inhalation group （group A） and normal control group （group D）.LTC4 and Th1/Th2 imbalance in bronchoalveolar lavage fluid （BALF） with all of the groups were compared after 7 days of inhalation.Results ① LTC4：compared with group A,B1,B2,C1,C2 and D groups were showing statistical significant difference （P 〈 0.01）; B1 and C1 groups showed statistical significant difference （P 〈 0.01）; B2 and C2 groups had statistical significant difference （P 〈 0.05）; B1 and B2 groups showed no statistical significant difference （P 〉 0.05）; ② Th1/Th2 imbalance：IFN-γ：compared with group A,B1,B2,C1,C2 and D groups were showing statistical significant difference （P 〈 0.01）; there were no statistical significant differences among the drug groups （P 〉 0.05）; IL-4：B1 and B2 groups had statistical significant difference （P 〈 0.01）; there were no statistical significant differences between B1 and C1 groups （P 〉 0.05）; there were no statistical significant difference between B2 and C2 groups （P 〉 0.05） ; IFN-γ/IL-4：B1 and B2 groups were showing statistical significant difference （P 〈 0.05）; there were no statistical significant difference between B1 and C1 groups （P 〉 0.05）; there were no statistical significant difference between B2 and C2 groups （P 〉 0.05）.Conclusion The rise of LTC4 and Th1/Th2 imbalance were important pathogenesises of bronchial asthma.Arsenic trioxide could relieve asthma by reducing LTC4 and adjusting the imbalance of Th1/Th2.Moxa leaf oil could relieve asthma by reducing LTC4.The combination bv inhaled smaller doses of As2O3 and moxa leaf oil was safe and effective to relieve asthma.There were drug synergisms between As2O3 and moxa leaf oil.