乙型肝炎肝纤维化患者血清转化生长因子1、白细胞介素10及干扰素γ的表达及临床意义

Clinical significance of the serum transforming growth facto-l, interleukin-lO, interferon-v in of patientswith hepatic fibrosis

目的探讨血清转化生长因子B1（TGF-B1）、白细胞介素10（IL-10）及干扰素1（INF．1）在肝纤维化患者中的变化及其与肝纤维化的相关性。方法选取2007—2013年在我院就诊的133例乙型肝炎肝纤维化患者为研究对象（观察组），分别对肝组织进行分期诊断，以酶联免疫吸附法对患者血清中的TGF-B1、IL-10及IFN-y的浓度进行测定，并选取21名健康体检者作为健康对照组。结果观察组血清TGF—b1及IL-10的浓度比健康对照组明显增高[血清TGF-131：（217．4±7．5）、（57．2±4．8）ng/L，f=15．61、P〈0．01；IL-10：（29．6±3．2）、（15．1士7．5）ng／L，t=11．44、P〈0．01]，而IFN-1的浓度则比健康对照组低[（51．9-I-3．5）、（75．8±5．9）ng／L，P=0．03]。血清TGF-B1、IL-10及IFN-1与肝纤维化分期之间存在一定的相关性，相关系数分别为0．786、0．644、-0．822，P均〈0．05。结论肝纤维化患者血清中的TGF-131、IL-10及INF-1的浓度对判别肝纤维化患者的发展趋势及预后具有较大的应用价值。

Objective To investigate the changes of serum transforming growth factor -B1 （TGF-131）, interleukin-10（IL-10） and interferon--y（INF-y） in elderly patients with hepatic fibrosis and their correlation with hepatic fibrosis. Methods One hundred and thirty-three patients diagnosed by pathological hepatic tissue were enrolled as case group who were in the first affiliated hospital of Xinxiang Medical University from 2007 to 2013. The enzyme-linked immunosorbent assay （ELISA） was applied to detect of serum levels of TGF-B1, IL-10 and IFN-y. Meanwhile 21 healthy people were recruited as control group. Results Comparison with the control group,the levels of TGF-B1 and IL-10 were significantly higher（TGF-B1 ： （217.4 ± 7. 5） ng/L vs. （57.2 ±4.8） ng/L,t = 15.61,P〈0.01;IL-lO：（29.6 ±3.2） ng/L vs. （15. 1 ±7.5） ng/L,t = 11.44,P 〈0.01）, while IFN-level was lower （ （51.9 ＋3.5） ng/L vs. （75.8 ±5.9） ng/L,P =0. 03） in case group. The serum levels of TGF-131, IL-IO, IFN-y and stages of hepatic fibrosis was correlated and the correlation coefficients wereO. 786,0. 644 and - 0. 822 respectively （P 〈 O. 05 ）. Conclusion Serum levels of TGF,131, IL-10 and IFN-yin patients with hepatic fibrosis are proved to be with ability of predicting the development of hepatic fibrosis and the post treatment.