原位PCR检测鸡传染性法氏囊病病毒

Detection of Infectious Bursal Disease Viruses by in situ PCR

采取临床病例鸡的法氏囊制成石蜡切片 ,经蛋白酶 K处理、原位 RT-PCR后 ,用地高辛精标记探针进行原位杂交检测传染性氏囊病病毒 ( IBDV)。结果 1 0个病例有 8个呈阳性 ,2个阴性。本试验尝试利用具有极高灵敏度的原位 PCR方法 ,从组织中检测低拷贝甚至单拷贝的目标序列 ,以鉴别隐性感染或混合感染 ,为IBDV的诊断和分子流行病学的分析提供了一种新的手段。

The bursae were harvested from chickens which were suspected to be infected with IBDV and paraffin embedded tissue sections were prepared.IBDV were detected by in situ RT PCR after these samples treated with protease K.The results showed that eight cases presented to be positive and two negative.We tried to detect low,even single target sequence for identification of silent or mixed infection by in situ PCR which the sensitivity is very high.It is practicable to apply in situ PCR on clinical diagnosis and molecular epidemiological investigation of IBDV.