禽脑脊髓炎的调查及病毒分离

Investigation and Virus Isolation of Avian Encephalomyelitis

对陕西省宝鸡、渭南等地的 2 4个鸡群进行了禽脑脊髓炎 ( AE)的调查 ,所调查鸡群都有产蛋下降史或产蛋正在下降 ,下降幅度平均为 1 7.2 %。用琼扩试验 ( AGP)检测所采集的 2 56份血清 ,AE阳性率平均高达85.6 %。有 1 0个鸡群阳性率达 1 0 0 %。跟踪调查 2个种鸡群 ,发现孵出的雏鸡从 3日龄开始发病 ,而且有 AE的症状 ,采发病雏鸡的脑组织 ,接种 6日龄鸡胚分离病毒 ,连传 3代 ,琼扩检测分离毒 ,呈 AE抗原阳性 ,人工感染 1日龄雏鸡复制出与原发病鸡群相同的疾例 ,表明分离毒是

Avian encephalomyelitis(AE) in 24 groups of chicken from Baoji and Weinan regions of Shaanxi province was investigated.All stocks involved had the history of the disease or a decreasing egg laying with average reducing extent of 17.2%. 256 serum samples from the stocks were tested with Agar Gel precipitin(AGP) test.It showed that 85.6% of the chickens were AE positive in average,especially 100% in 10 stocks.Further detection in 2 groups of breeding chickens indicated that the chicken appeared the clinical symptoms of AE in 3 days after hatched.The brain sample preperations from the sick chicken were injected into chicken embryos to isolate the virus.The virus collected from the third generation were tested to be positive for AE using AGP test. 1 day chickens inoculated with the isolates revealed the same symptoims as the natural disease. The result indicated that the isolate should be the AE virus.