细胞及瘤块种植人前列腺癌裸鼠皮下移植瘤模型的比较

Comparison of subcutaneously xenografts model of human prostate cancer in nude mice established by cell suspension and tumor tissue

目的比较细胞悬液法及瘤块法种植裸鼠前列腺癌皮下移植瘤,以期获得使瘤体生长最快,质量最佳的种植方式。方法采用细胞悬液及瘤块两种方式种植PC-3细胞人前列腺癌皮下移植瘤,比较两种种植方法所种植的移植瘤的成瘤率、成瘤潜伏期、瘤体形态以及移植瘤的中心坏死灶,同时对两种方法种植的皮下移植瘤进行常规病理学检查。本研究同时观察了PC-3细胞悬液浓度对于种植皮下移植瘤成瘤的影响。结果两种方式种植的移植瘤的成瘤率差异无统计学意义,采用瘤块种植的移植瘤生长较细胞种植稍快,瘤体形态各异,较容易发生中心性坏死;细胞悬液组成瘤稍慢,瘤体较规则,多呈圆形或椭圆形,发生中心性坏死较晚,而且随着细胞浓度增高,其成瘤潜伏期明显缩短。结论通过比较发现细胞悬液种植皮下移植瘤较瘤块种植更有优势,而且采用较高浓度细胞悬液种植可以有效缩短成瘤潜伏期,是一种较为理想的种植方式。

Objective To compare the different method of transplanting subcutaneously transplanted tumor in nude mice.Methods Xenograft models of human PC-3 prostate cancer in nude mice with cell suspension of different concentrations and tumor tissue were established,respectively.Then the time of tumor formation,take rates,morphological features and central tumor necrosis werecompared between the two groups.Meanwhile the histopathological features of transplanted tumor were examined,the tumor volume were measured,and the growth curve was drawn.Results No significant differences were observed in take rates in tumors established from the two types of implants.The xenograft of tumor tissue group formed in an earlier time,but the shape was irregular and central necrosis occured more easily.Though the xenograft of cell suspension group formed later than tumor tissue group,the shape was regular and central necrosis occurred late.There were no significant differences in the pathological features between the two groups.With the increase of concentrations of cells,the time of tumor formation was significantly shortened.Conclusion High concentration cell suspension is an ideal method to implant subcutaneously transplanted tumor of prostate cancer in nude mice.