凝血因子Ⅶ水平及其基因多态性与PTE的相关研究

Correlation of coagulation factor Ⅶ gene polymorphism and its level to pulmonary thromboembolism

目的探讨FⅦ基因R353Q多态性及血浆FⅦa水平与PTE的相关性。方法收集肺血栓栓塞症(PTE)患者198例和对照组212例;采用PCR-PFLP检测FⅦ基因R353Q多态性,ELISA法测定血浆FⅦa水平。结果①FⅦ基因R353Q单核苷酸多态性(SNP)基因分布频率和等位基因携带频率在病例与对照组差异均无统计学意义(P>0.05)。②血浆FⅦa水平PTE组与对照组比较,差异有显著性意义(P=0.000)。③通过非条件Logistic回归模型校正后,吸烟、纤维蛋白原和血浆FⅦa水平是PTE患者的独立危险因素。结论高血浆FⅦa水平是PTE患者的独立危险因素;FⅦ基因R353Q多态性可能不是PTE患者的独立危险因素。

Objective To investigate the correlation of coagulation factor FⅦ gene R353Q mutation and its plasma level to pulmonary thromboembolism （PTE）. Methods The study selected 198 patients with PTE as the ob- servation group and 212 healthy people as controls. Polymerase chain reaction-restriction fragment length polymor- phism （PCR-RFLP） was applied to detect the polymorphism of FⅦ gene R353Q and enzyme-linked immune sorbent assay （ELISA） was adopted to determine the plasma level of F Ⅶa. Results ① There was no significant difference in the SNP genotyping distribution frequency and the R/Q-allele frequency between the two groups （ P 〉 0. 05 ）. ② The plasma level of FⅦa was significantly higher in the observation group than in the controls （P = 0. 000）. ③ Multifactor logistic regression analysis showed that plasma FⅦa level, fibrinogen and smoking were independent risk factors for PTE patients. Conclusion High plasma F Ⅶ a level is a common risk factor for PTE patients. The F Ⅶ R353Q polymorphism may not be an independent risk factor for PTE patients.