鲍鱼性腺小肽的制备及抗氧化活性的初步研究

Antioxidant Activity of Enzymatic Hydrolysates Prepared from Pacific Abalone(Haliotis discus hannai) Gonads

以雄性皱纹盘鲍性腺为原料,建立了蛋白酶酶解制备抗氧化活性蛋白肽的工艺条件.分别对木瓜蛋白酶和中性蛋白酶进行单因素研究,并以二者的复合酶为工具酶进行响应面分析.结果显示,雄性鲍鱼性腺的最优酶解条件为木瓜蛋白酶与中性蛋白酶的比例为1∶4、pH=6.6、酶解温度为53.7℃、酶解时间为70.4 min.高效液相色谱(HPLC)分析结果表明,酶解液中多肽的分子质量主要分布在1 ku以下.制备的酶解液具有较强的清除自由基效果,清除1,1-二苯基-2-三硝基苯肼(DPPH)的EC50值为6.8 mg/mL,还原力的AC0.5值为12.87 mg/mL.

Using male abalone gonads as raw material, the present study optimized the proteinase hydrolyzing conditions to produce antioxidant peptides. Single-factor experiments using papain and neutral proteinase were adopted respectively and the combination of both enzymes was performed. The results showed that the optimal hydrolyzing conditions for preparation of active antioxidant peptides from male abalone gonads were using the proportion of papain to neutral proteinase at 1 ： 4, at pH = 6. 6 and 53.7 ℃, with hydrolyzing duration of 70. 4 min. High performance liquid chromatography （HPLC） analysis showed that the molecular mass of peptides in the hydrolysate was mostly below 1 ku. The hydrolysate thus obtained from male abalone gonads revealed a significant effect in scavenging free radicals. The half scavenging effect （EC50） of DPPH radical was 6. 8 mg/mL and the half absorbanee effect （AC0.5） of reducing power was 12. 87 mg/mL.