GSTT1基因多态性及吸烟与胃癌的关系

Relationship of GSTT1 genotypes and smoking on the susceptibility to gastric cancer

目的 研究谷胱甘肽转硫酶GSTT1基因缺失多态性及吸烟情况与胃癌易感性的关系.方法 研究对象为2005年5月～2006年3月在包头地区确诊的胃癌病例（60例）;以同期该地的排除肿瘤病史的一般人群（83例）为对照.采用病例-对照研究的方法,调查研究对象的吸烟情况,以聚合酶链反应（PCR）的技术方法分析GSTT1的基因型.用SPSS 13.0软件分析研究结果.结果 ①GSTT1（-）基因型的频率在胃癌组和对照组分别为56.7％和39.8％,差异有统计学意义（P＜0.05）;GSTT1（-）者发生胃癌的危险性较GSTT1（＋）者显著升高（OR=1.98,95％CI：1.01～3.89）.②吸烟者的比例在胃癌组和对照组分别为65.0％和34.9％,组间比较差异有统计学意义（P＜0.05,OR=3.458,95％CI：1.723～6.939）.③通过两组分层分析,发现GSTT1（-）基因人群中,吸烟者发生胃癌的风险比非吸烟者高（P＜ 0.05,OR=11.6,95％CI：3.516～28.266）;吸烟人群中,GSTT1（-）基因者发生胃癌的风险比GSTT1（＋）者高（P＜ 0.05,OR=4.75,95％CI：1.68～13.41）.结论 GSTT1基因缺失多态性与胃癌易感性有关;GSTT1基因缺失及吸烟在胃癌的发生中有协同作用.

Objective To analyze GSTT1 gene polymonphism,smoking and their effects on susceptibility to gastric cancer.Methods The objects consisted of 60 gastric cancer patients from May 2005 to March 2006 in Baotou City,in comparison with 83 population-based controls without tumor.The epidemiological data （smoking） was obtained from all of the above subjects.GSTT1 genotype was determined by a multiplex PCR.The results were analyzed by SPSS 13.0.Results ①The distribution frequency of GSTT1 （-） genotype （56.7%） among the gastric cancer patients was more than the control group （39.8%） （P 〈 0.05）.In people with GSTT1 （-） genotype,the risk for gastric cancer was higher than the GSTTl （＋） genotype （OR =1.98,95%CI：1.01-3.89）.②The frequency of smokers （65.0%） among the gastric cancer patients was more than the control group （34.9%） （P 〈 0.05,OR =3.458,95%CI：1.723-6.939）.③In people with GSTT1 （-） genotype,smoking markedly increased the risk for gastric cancer （P 〈 0.05,OR =11.6,95%CI：3.516-28.266）; among the smokers,GSTT1 （-） genotype significantly increased the risk for gastric cancer （P 〈 0.05,OR =4.75,95%CI：1.68-13.41）.Conclusion The GSTT1 gene deletion is significantly related to the susceptibility to gastric cancer; the GSTT1 gene deletion and smoking have interaction on gastric cancer.