两个转W23基因小麦株系的抗旱性鉴定

Identification of drought resistance of two transgenic wheat lines with W23 gene

以两个T5代转W23基因小麦株系G19-X59、G19-X61为材料,在水培条件下采用PEG-6000人工模拟干旱胁迫措施对转基因小麦株系的根系发育特点、抗旱相关的光合生理等指标进行了测定。结果表明,干旱胁迫条件下各参试小麦的光合速率(Pn)和蒸腾速率(Tr)均下降,转基因株系降幅较小,且其Pn和Tr值极显著高于受体品种;在正常供水和干旱胁迫两种水分处理下,转基因株系均比受体品种具有较发达的根系,其根总长、根总表面积、根总体积等参数极显著高于受体品种。这一结果说明,转W23基因小麦株系G19-X59、G19-X61在苗期依靠发达的根系维持较强的光合作用,提高其应对干旱胁迫的能力。

Two T5 transgenic wheat lines G19-X59 and G19-X61 with W23 gene were used as materials, 15% PEG-6000 was used to simulate the drought stress in hydroponic culture, the root development characteristics of transgenic wheat lines and photosynthetic physiology indexes related to drought resistance were investigated. The results indicated that photosynthetic rate （Pn） and transpiration rate （Tr） of all the wheat materials decreased under drought stress, whereas those of the transgenic wheat lines were not significantly changed. The Pn and Tr values of transgenic wheat lines were extremely significantly higher than that of the control Jimai 19. The transgenic lines were also found to have more developed root system than the control under both normal water supply and drought stress treatments. There were also extremely significant difference in total root length, root surface area and root volume between transgenic lines and the control. The results showed that the transgenic lines G19-X59 and G19-X61 could maintain high level of photosynthesis and thereby have strong resistance to drought stress.