外源性抗坏血酸消除红细胞中高铁血红蛋白的ESR研究

Electron Spin Resonance Studies on Inhibiting Methemoglobin in Erythrocyte with Exogenous Ascorbic Acid

研究外源性抗坏血酸对红细胞中高铁血红蛋白的消除作用 ,有助于深入了解外源性抗氧化剂在红细胞中的血红蛋白发生红细胞自身难以消除的氧化变性时的效力。利用电子自旋共振 (ESR)技术对高铁血红蛋白中Fe3 + 灵敏的分析能力 ,我们进行了血红蛋白氧化变性和外源性抗坏血酸对红细胞中高铁血红蛋白消除作用的研究。研究结果显示 :没有加入外源性抗坏血酸的血液样品 ,在加入NaNO2 后 ,5、35、90min的ESR检测 ,均发现高自旋的高铁血红蛋白的吸收峰 (g =6 ) ,该峰随时间而增大。加入外源性抗坏血酸的血液样品 ,在加入NaNO2 后 ,5、35、90min的ESR检测 ,只在 35min的检测中发现高自旋的高铁血红蛋白的吸收峰 (g=6 )。我们的研究结果提示 :外源性抗氧化剂对红细胞自身难以消除的血红蛋白氧化变性有抑制和消除作用。

Research on inhibiting the methemoglobin(MetHb)in erythrocyte by means of exogenous ascorbic acid is helpful to elucidating whether exogenous antioxidant inhibits the oxidative denaturation of hemoglobin when the erythrocyte does not inhibit such denaturation with its own antioxidation system. Using electron spin resonance(ESR) technique, we have studied the oxidative denaturation of hemoglobin and the inhibition of MetHb in erythrocyte with the exogenous ascorbic acid. The results indicate that there is the ESR absorption of high spin MetHb(g=6) at the 5th, 35th and 90th min after the blood was mixed with NaNO 2. The ESR absorption of high spin MetHb increases with time. Yet, in the case where the blood was mixed with exogenous ascorbic acid in advance, the ESR absortpion of high spin MetHb(g=6) only appears at the 35th min among the ESR measures at the 5th, 35th, and 90th min after the blood was mixed with NaNO 2. These findings suggest that exogenous antioxidant can inhibit the oxidative denaturation of hemoglobin when the erythrocyte does not inhibit such dematuration with its own antioxidation system.