青海省小麦品种阿勃高分子量麦谷蛋白遗传多样性

Genetic Diversity of HMW-GS in Wheat Abodanza from Qinghai

为了研究小麦品种阿勃在青海省不同生态区种植的广适性，本试验采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS—PAGE）技术，以青海省不同生态区15份阿勃为材料进行高分子量麦谷蛋白亚基（HMW．GS）的遗传多样性分析。结果表明：阿勃HMW—GS的亚基组合类型有8种，主要为1／7＋8／2＋12；有11种亚基，各个亚基出现的频率范围6．67％-100％；Glu-B1位点遗传多样性指数较大，为0．239；供试材料群体间Gs的变化范围为O．35—1．00，平均为0．675，所有阿勃聚为3类，其中13份阿勃材料聚在第1类，另2份分别聚在第Ⅱ和第Ⅲ类，说明在青海不同生态区本土种植保留下的阿勃HMW．GS遗传多样性丰富。

In order to study the wide adaptation and detect the genetic diversity of Abodanza （ Triticum. aestirum L. ）in different ecological areas of Qinghai, HMW-GS subunit compositions of 15 Abodanza materials were analyzed by SDS-PAGE. The results described that a total of 8 allele combinations were observed in the 15 Abodanza materials and the predominant type was 1,7 ＋ 8, and 2 ＋ 12. There were 11 alleles on Glu-1 locus with the frequen- cies varied from 6.67% to 100%. The D values of the Glu-B1 loci were higher. The result showed that genetics similarities ranged from 0.35 to 1 with an average of 0. 675. All materials were divided into 3 groups and 13 wheat Abodanza materials were clustered into the first group while another two materials were respectively clustered into the second group and the third group. These results indicated that Abodanza materials had abundant genetic diversity.