羊水与自体血培养后刺激花生四烯酸代谢物的释放

Release of arachidonic acid metabolites from blood by cultivation of human amniotic fluid with oneself blood

目的 :探讨人羊水在体外刺激自体血细胞释放前列环素 (PGI2 )、血栓素A2 (TXA2 )和白三烯C4(LTC4 )等花生四烯酸代谢物的作用。方法 :取产妇羊水与自体血进行培养 ,用放射免疫分析法检测血中血栓素B2 (TXB2 )和 6 -酮前列腺素F1α(6 -Keto -PGF1α)的含量 ,用酶联免疫法检测LTC4 。结果 :羊水能刺激血细胞释放TXA2 和LTC4 ,胎粪污染的羊水作用更为明显。TXB2 的含量由加羊水培养前的 (6 3.5± 5 2 .0 )ng/L增加到培养后的 (189.1± 10 2 .0 )ng/L(P <0 0 1) ,用胎粪污染羊水与血培养后增加到 (2 89.2± 113.2 )ng/L(P <0 0 1) ;LTC4 的含量由培养前的 (4 0 .1± 39.7)ng/L增加到培养后的 (2 93.5± 2 0 6 .1)ng/L(P <0 0 1) ,胎粪污染组增加到(387.2± 2 14.6 )ng/L(P <0 0 1) ,但前列环素仅有轻度增加 ,无显著性差异 (P >0 0 5 )。结论 :羊水能刺激血细胞释放花生四烯酸类生物活性物质 ,使其正常的平衡状态被破坏 ,可能与羊水栓塞的发生机理有关。

AIM: To investigate the effect of human amniotic fluid on the release of thromboxane A 2 (TXA 2), prostaglandin I 2 (PGI 2) and Leukotriene C 4(LTC 4) from blood cells. METHODS: 1 mL human amniotic fluid and 10 mL oneself blood collected from 38-41 weeks with cesarean section were cultured at 37℃ for 30 min, and then centrifuged. The supernatants were taken and stored at -70℃. TXB 2 and 6-Keto-PGF 1α of the superntants were determined by radioimmunoassay and LTC 4 by enzyme immunoassay. RESULTS: It was found that the levels of TXB 2 and LTC 4 in blood were elevated from (63.5±52.0) ng/L and (40.1±39.2) ng/L to (189.1±102.0) ng/L and (293.5±206.1) ng/L respectively (P<0.01) after cultivation of oneself amniotic fluid with blood, the concentrations of TXB 2 and LTC 4 were higher in meconium-stained group than those in clear amniotic fluid group, but the concentration of PGI 2 only elevated slightly from (27.4±11.6) ng/L to (33.9±10.6) ng/L(P>0.05).CONCLUSION: Amniotic fluid might stimulate the release of TXA 2 and LTC 4 from blood, it might affect the balance of TXA 2 and PGI 2 in blood, which might play an important role in the pathogenesis of amniotic fluid embolism.