小柴胡汤对糖皮质激素受体及其mRNA的调节作用

Regulation of glucocorticoid receptor and its mRNA by Tsiao Shihutang

目的和方法 :给予健康雄性小鼠 (Balb/C)小柴胡汤 (TSS) ,糖皮质激素 (GC) ,应用受体放射配体结合分析法测定小鼠脾细胞糖皮质激素受体 (GR)位点数 ,同时用定量逆转录 多聚酶链反应 (RT PCR)法测定脾细胞内GRmRNA水平 ,以期从分子受体水平和基因水平探讨TSS对GR及GRmRNA的调节作用。结果和结论 :(1)应用TSS后GR位点数及GRmRNA水平明显少于正常对照组 ,说明TSS对GR的降调是通过调节GRmRNA水平实现的 ;(2 )TSS与GC合用时GR位点数及GRmRNA水平明显高于单独应用GC组 ,说明TSS可明显减弱GC对GR的降调作用。

AIM: In order to explore the mechanism of the GC-like effect of Tsiao Shaihutang (TSS), the down-regulation of glucocorticoid receptor and mRNA by TSS were studied.METHODS: GR sites were determined by receptor radio ligand binding assay method. At the same time, GR mRNA level was determined by quantitative reverse-transcriptive polymerase chain reaction (RT-PCR) method.RESULTS: (1) GR sites and GR mRNA level were down-regulated significantly after GC, TSS (P<0.01) treatment . (2) GR sites and GR mRNA level in GC plus TSS group were obviously higher than those in GC group (P<0.01).CONCLUSIONS: These data suggested that TSS can significantly down-regulate GR and GR mRNA level.