神经肽Y对bcl-2,bax,fas基因表达和血管平滑肌细胞增殖的作用

Effect of neuropeptide Y on bcl-2, bax, fas expressions and proliferation in vascular smooth muscl cells

目的 :研究神经肽Y(NPY)对细胞凋亡相关基因表达和细胞增殖的作用。方法 :应用生物化学比色法和荧光免疫组化定量技术观察NPY对血管平滑肌细胞 (VSMC)中细胞凋亡相关基因bcl - 2 ,bax及fas表达和细胞核增殖抗原 (PCNA)的平均荧光值。结果 :NPY作用下 ,细胞凋亡相关基因bcl- 2 ,bax和fas表达及VSMC的增殖活度和PCNA明显高于对照组。结论

AIM:To explore the effect of neuropeptide Y on expression of apoptosis associated genes and proliferation in vascular smooth muscle cells(VSMC). METHODS: The proliferation activity of VSMC was dterminded by MTT colorimetry. The average fluorescence intensity that represented VSMC nuclear antigen (PCNA) and bcl-2, bax, fas expressions was quantitatively measured by fluorescence immunohistochemistry. RESULTS: Compared with control, the expressions of bcl-2, bax, fas, PCNA and the VSMC proliferation activity in VSMC treated with NPY were significantly increased. CONCLUSION:NPY may increase the expression of apoptosis associated genes in VMSC and promote its proliferation.