高效毛细管电泳法测定甘草栓中甘草酸和甘草次酸的含量

Determination of Glycyrrhizic Acid and Glycyrrhetinic Acid in Suppositoria Radix Glycyrrhizae by High Performance Capillary Electrophoresis(HPCE)

目的 :建立甘草栓中甘草酸、甘草次酸的含量测定新方法 ,为中药甘草制剂的质量控制提供新的检测方法。方法 :采用高效毛细管电泳法同时测定主要成分。电解缓冲液由 2 5mmol/L硼砂溶液和 10 0mmol/L十二烷基磺酸钠 (SDS)溶液组成。熔融石英毛细管 75 μm× 6 0cm ,在 2 0kV电压下运行 ,进样时间 10s,检测波长为 2 5 4nm ,温度 2 5℃。 结果 :上述条件下 ,甘草酸、甘草次酸得到较好分离 ,加样回收率分别为 99.2 %和 99.7%,RSD分别 2 .3 %和 2 .6 %。天间和天内精密度RSD在 1.0 0 %- 3 .0 0 %(n =3)。线性浓度范围在 2 5 - 30 0 μg/ml。 结论 :该方法简便、快捷、准确 ,具良好的精密度和回收率 ,可作为中药甘草制剂的质量控制方法。

Objective: To establish a new method for determination of glycyrrhizic acid and glycyrrhetinic acid in Suppositoria Radix Glycyrrhizae. Methods: The main components were determined by HPCE, and which buffer consisted of 25mmol/L borate phosphate (pH 10) and 100mmol/L sodium dodecyl sulfate (SDS);I eff =50cm, L=60cm, i.d.=75μm,V=20kV, sampling time=10s, λ=254nm, T=25℃. Results: The recoveries of glycyrrhizic acid and glycyrrhetinic acid were 99.2% and 99.7%, respectively. The relative standard deviations (RSD) were 2.3% and 2.6%, respectively. The precision RSDs of within day and between day lied at the range of 1.00% to 3.00%. The linear calibration range was from 25 to 300μg/ml.Conclusion: This method is simple, accurate and reproducible and can be used for the quality control of Chinese herbal medicines with good precision and recoveries.