慢性应激对大鼠血清活性肾素与血管紧张素Ⅱ的影响及电针的干预作用

Effect of Chronic Stress Depression in Serum Active Renin and Angiotensin Ⅱ and Effect of electro-acupuncture in it

目的观察慢性应激抑郁模型大鼠血清活性肾素与血管紧张素Ⅱ的变化以及电针的干预作用,探讨肾素-血管紧张素系统在抑郁病与心血管疾病之间的关联。方法采用慢性应激刺激结合孤养的方式建立大鼠慢性应激抑郁模型,分组后分别采用电针、氟西汀治疗21 d,通过敞箱试验观察大鼠的行为学变化;采用ELISA检测各组大鼠血清活性肾素和血管紧张素Ⅱ水平。结果模型组大鼠敞箱试验水平运动得分和垂直运动得分明显降低,电针和氟西汀可逆转此变化;模型组大鼠血清中活性肾素水平和血管紧张素Ⅱ的水平升高,电针和氟西汀均可降低两者的变化。结论慢性应激抑郁模型大鼠的循环RAS系统被过度激活,电针和氟西汀可降低其激活程度。

Objective： To observe the changes of serum active renin and Angiotensin II in chronicity stress depression model rat and the interference of electroacupuncture to them so as to explore the function of the reninangiotensin system between depression and cardiovascular disease. Methods： The chronic unpredictable mild stress combined with solitarily feeding method was used to make the depression model. The locomotor activity of rats in blank control group, model control group, electro-acupuncture group and fluoxetine group were observed by open-field experiment. Serum active renin and Ang II levels were determined by using enzyme-linked immunoab- sorbent assay. Results： The cross and rear scores of the model group were significantly lower than those of the blank group,while electro-acupuncture and fluoxetine reversed it. Levels of active renin and Ang II in serum in model group were higher than those in the control group. Electro-acupuncture and fluoxetine were obvious to improve those pathological changes. Conclusion： Circulatory pisma RAS are activated excessive in chronicity stress depression model rats. The electro-acupuncture and fluoxetine can improve those pathological changes.