可活化诱导的淋巴细胞凋亡在乙型肝炎慢性化机制中的作用

Role of activation induced apoptosis of lymphocytes in the persistence of hepatitis B virus infection

目的 探讨可活化诱导的淋巴细胞凋亡在慢性乙型肝炎 (乙肝 )发病机制中的作用。方法 应用流式细胞仪对乙肝外周血淋巴细胞的凋亡进行检测 ,乙型肝炎病毒 (HBV)DNA定量检测采用斑点杂交法。结果 与正常对照组比较 ,可活化诱导的淋巴细胞凋亡在慢性乙肝患者明显升高 ,而在重型肝炎则降低 ,差异有显著性 ;急性肝炎则无。可活化诱导的淋巴细胞凋亡在丙氨酸转氨酶(ALT)下降期明显高于上升期 ,但与肝内炎症分级并无明显相关性。乙肝外周血淋巴细胞的凋亡比例与血清HBVDNA含量呈正相关。结论 (1)可活化诱导的淋巴细胞凋亡可能参与乙肝的慢性化 ;(2 )

Objective To investigate the role of activation induced apoptosis of lymphocytes in the pathogenesis of chronic hepatitis B. Methods The apoptosis of peripheral lymphocytes extracted from chronic hepatitis B patients were analyzed by flow cytometer. HBV DNA was detected quantitatively by dot blot hybridization. Results Compared with the control, the percentage of apoptosis cells was significantly higher in the patients with chronic hepatitis B and lower in severe hepatitis. Apoptosis could not be observed in acute hepatitis patients. Activation induced lymphocyte apoptosis was more obvious in ALT declining phase than in uprising phase, but it has no relationship with the inflammation stage in liver. However there was positive relationship between serum HBV DNA load and the percentage of apoptosis cells. Conclusions (1) Activation induced apoptosis of lymphocytes might be related with the persistence of hepatitis B virus infection. (2) The levels of serum HBV DNA can affect the apoptosis of peripheral lymphocytes.